Superior Institute of Biomedical Sciences, Ceará State University, Fortaleza, Ceará, Brazil.
Superior Institute of Biomedical Sciences, Ceará State University, Fortaleza, Ceará, Brazil..
Res Vet Sci. 2023 Aug;161:163-172. doi: 10.1016/j.rvsc.2023.06.020. Epub 2023 Jun 28.
Endothelial dysfunction is an early complication of diabetes and it is related to both micro- and macroangiopathies. In addition, >70% of diabetic patients develop autonomic neuropathies. Increased oxidative stress has a major role in the development of both nitrergic and endothelial dysfunction. The aim of this work is to evaluate whether rutin, a potent antioxidant, could ameliorate nitrergic and/or endothelial dysfunction in diabetic animals. Primary and secondary treatment protocols with rutin were investigated on rat aortic rings and the mesenteric arteriolar bed, and on rabbit aortic rings and corpora cavernosa (RbCC) from both euglycemic and alloxan-diabetic animals. Acetylcholine endothelium-dependent and sodium nitroprusside endothelium-independent relaxations were compared in tissues from euglycemic or diabetic animals. Electrical field stimulation (EFS)-induced relaxation was performed only in the RbCC. Endothelial-dependent relaxations were blunted by 40% in vessels and neuronal relaxation was blunted by 50% in RbCC taken from diabetic animals when compared to euglycemic animals. Pre-treatment with rutin restored both neuronal and endothelial dependent relaxations in diabetic animals towards the values achieved in control euglycemic tissues. Rutin was able to ameliorate both endothelial dysfunction and nitrergic neuropathy in animal experimental models. Rutin could be a lead compound in the primary or secondary preventive ancillary treatment of endothelial and nitrergic dysfunction in the course of diabetes.
内皮功能障碍是糖尿病的早期并发症，与微血管和大血管病变均有关。此外，超过 70%的糖尿病患者会发展出自发性神经病变。氧化应激增加在氮能和内皮功能障碍的发展中起着主要作用。这项工作的目的是评估芦丁，一种有效的抗氧化剂，是否可以改善糖尿病动物的氮能和/或内皮功能障碍。在大鼠主动脉环和肠系膜小动脉床以及正常血糖和链脲佐菌素糖尿病兔的主动脉环和海绵体（RbCC）上研究了芦丁的原发性和继发性治疗方案。在正常血糖或糖尿病动物的组织中比较了乙酰胆碱内皮依赖性和硝普钠内皮非依赖性松弛。仅在 RbCC 上进行电刺激诱导的松弛。与正常血糖动物相比，糖尿病动物的血管内皮依赖性松弛降低了 40%，RbCC 的神经元松弛降低了 50%。芦丁预处理可使糖尿病动物的神经元和内皮依赖性松弛恢复到对照正常血糖组织的水平。芦丁能够改善动物实验模型中的内皮功能障碍和氮能神经病变。芦丁可能是糖尿病过程中内皮和氮能功能障碍的初级或二级预防辅助治疗的先导化合物。
