Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region & School of Life Sciences, Heilongjiang University, Harbin 150080, China.
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region & School of Life Sciences, Heilongjiang University, Harbin 150080, China; Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province, Heilongjiang University, Harbin 150080, China; Hebei University of Environmental Engineering, Hebei Key Laboratory of Agroecological Safety, Qinhuangdao 066102, China.
Bioresour Technol. 2023 Oct;385:129451. doi: 10.1016/j.biortech.2023.129451. Epub 2023 Jul 3.
In this study, 16S rRNA sequencing and GC-MS (gas chromatography-mass spectrometry) techniques were employed to examine the relationship between bacterial succession and metabolite alterations during the dew retting process of flax. The results indicated that the addition of compound microbial agents may affect the production and transformation of metabolites by re-establishing bacterial communities and promoting the degradation of pectic substances and the release of metabolites, and the best retting effect was achieved under the combined addition (BA). In addition, Chryseobacterium, Bacillus, and Pseudoonas were closely associated with the production of fatty acids and alcohols; the addition of compound microbial agents increased the content of critical metabolites while decreasing the environmental pollutant bis(2-ethylhexyl) phthalate. In summary, the addition of compound microbial agents can positively regulate the retting process of flax, shorten the retting cycle, improve the quality of flax fibre, and reduce the pollution of the environment.
在这项研究中，采用 16S rRNA 测序和 GC-MS（气相色谱-质谱）技术研究了亚麻雨露沤麻过程中细菌演替与代谢物变化的关系。结果表明，添加复合微生物制剂通过重建细菌群落和促进果胶物质的降解和代谢物的释放，可能会影响代谢物的产生和转化，并且在联合添加（BA）的情况下可以达到最佳沤麻效果。此外，金黄杆菌、芽孢杆菌和假单胞菌与脂肪酸和醇类的产生密切相关；添加复合微生物制剂增加了关键代谢物的含量，同时降低了环境污染物邻苯二甲酸二（2-乙基己基）酯的含量。总之，添加复合微生物制剂可以正向调节亚麻沤麻过程，缩短沤麻周期，提高亚麻纤维的质量，并减少对环境的污染。
