Erol Ümit Haydar, Güncüm Enes, Işıklan Nuran
Department of Chemistry, Faculty of Arts and Sciences, Kırıkkale University, Yahşihan, 71450, Kırıkkale, Turkey; Advanced Technology Application and Research Center, Kilis 7 Aralık University, 79000 Kilis, Turkey.
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Kırıkkale University, 71450 Yahşihan, Kırıkkale, Turkey.
Int J Biol Macromol. 2023 Aug 15;246:125627. doi: 10.1016/j.ijbiomac.2023.125627. Epub 2023 Jul 3.
The use of natural polymeric nanoparticles (Nps) as drug carriers is a highly promising area of research in the field of drug delivery systems because of their high efficiency. In this study, flurbiprofen (FB) loaded chitosan-graphene oxide (CS-GO) blend Nps were synthesized as a controlled delivery system using the emulsion method. The crystalline, molecular, and morphological structures of the prepared CS-GO Nps were characterized using a variety of analytical methods, including Fourier transform infrared (FT-IR) spectroscopy, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), X-Ray diffractometry (XRD), scanning electron microscopy (SEM), and atomic force microscopy (AFM). It was found that the introduction of GO into the CS nanoparticle formulation increased its thermal stability. The range of the average particle size was between 362 ± 5.06 and 718 ± 2.21 nm, with negative zeta potential values between -7.67 ± 4.16 and - 27.93 ± 2.26 mV. The effects of the CS/GO ratio, the FB/polymer ratio, the amount of span 80, and the cross-linker concentration were assessed on FB release profiles. In vitro release studies displayed a two-stage release behaviour with a fast initial release of the FB, followed by sustained and extended release, and the incorporation of GO into the CS Nps made the FB release more sustained and controlled manner. Besides, the cytotoxicity test of the FB-loaded CS-GO Nps was studied through MTT assay, and it was found that they were biocompatible. Based on these findings, it can be inferred that the prepared CS-GO Nps might be a promising candidate drug carrier system for FB.
由于其高效率，使用天然聚合物纳米颗粒（Nps）作为药物载体是药物递送系统领域中一个非常有前景的研究领域。在本研究中，采用乳液法合成了负载氟比洛芬（FB）的壳聚糖 - 氧化石墨烯（CS - GO）混合纳米颗粒作为控释系统。使用多种分析方法对制备的CS - GO纳米颗粒的晶体、分子和形态结构进行了表征，包括傅里叶变换红外（FT - IR）光谱、差示扫描量热法（DSC）、热重分析（TGA）、X射线衍射法（XRD）、扫描电子显微镜（SEM）和原子力显微镜（AFM）。研究发现，将氧化石墨烯引入CS纳米颗粒配方中可提高其热稳定性。平均粒径范围在362±5.06至718±2.21nm之间，zeta电位负值在 - 7.67±4.16至 - 27.93±2.26mV之间。评估了CS/GO比例、FB/聚合物比例、span 80用量和交联剂浓度对FB释放曲线的影响。体外释放研究显示出两阶段释放行为，FB最初快速释放，随后是持续和延长释放，并且将氧化石墨烯掺入CS纳米颗粒中使FB释放更具持续性和可控性。此外，通过MTT法研究了负载FB的CS - GO纳米颗粒的细胞毒性试验，发现它们具有生物相容性。基于这些发现，可以推断所制备的CS - GO纳米颗粒可能是FB的一种有前景的候选药物载体系统。
