Department of Chemistry, Faculty of Arts and Sciences, Kırıkkale University, 71000 Kırıkkale, Turkey.
Department of Chemistry, Faculty of Arts and Sciences, Kırıkkale University, 71000 Kırıkkale, Turkey; Advanced Technology Application and Research Center, Kilis 7 Aralık University, 79000 Kilis, Turkey.
Int J Biol Macromol. 2020 Sep 15;159:751-762. doi: 10.1016/j.ijbiomac.2020.05.071. Epub 2020 May 13.
In present work, temperature-responsive flurbiprofen (FLU) containing chitosan/hydroxypropyl cellulose (CS/HPC) blend nanospheres were prepared using emulsion method. The structures of blend nanospheres were characterized by ATR-FTIR, XRD, SEM, DSC/TGA, zeta potential and particle size analyses. Their lower critical solution temperatures (LCST) were determined and found to be 42 °C. In vitro release studies were performed in gastrointestinal-tract simulated conditions at 30 °C, 37 °C and 44 °C. As the medium temperature was increased, the release of FLU decreased, indicating that blend nanospheres had temperature-responsive feature. The FLU release demonstrated that release profiles depend upon CS/HPC ratio, amount of FLU present in the nanospheres and percentage of cross-linker used. Moreover, the cytotoxicity tests were performed via MTT method and it was observed CS/HPC nanospheres were biocompatible. Based on the in vitro release profile and cytotoxicity studies, the fabricated CS/HPC blend nanospheres could be a promising candidate as a temperature-responsive nano-carrier for controlled drug release.
在本工作中，采用乳化法制备了载有温度响应性氟比洛芬（FLU）的壳聚糖/羟丙基纤维素（CS/HPC）共混纳米球。通过 ATR-FTIR、XRD、SEM、DSC/TGA、ζ 电位和粒径分析对共混纳米球的结构进行了表征。测定了它们的低临界溶液温度（LCST），发现为 42°C。在 30°C、37°C 和 44°C 的胃肠道模拟条件下进行了体外释放研究。随着介质温度的升高，FLU 的释放减少，表明共混纳米球具有温度响应特性。FLU 的释放表明，释放曲线取决于 CS/HPC 比、纳米球中存在的 FLU 量和使用的交联剂百分比。此外，通过 MTT 法进行了细胞毒性试验，观察到 CS/HPC 纳米球具有生物相容性。基于体外释放曲线和细胞毒性研究，所制备的 CS/HPC 共混纳米球可以作为一种有前途的温度响应性纳米载体，用于控制药物释放。
