College of Life Sciences, Hebei Agricultural University, Baoding, 071001, China.
College of Horticulture, Hebei Agricultural University, Baoding, 071001, China.
BMC Genomics. 2023 Jul 5;24(1):377. doi: 10.1186/s12864-023-09492-1.
Basic leucine zipper (bZIP) proteins are important transcription factors in plants. To study the role of bZIP transcription factors in walnut explant browning, this study used bioinformatics software to analyze walnut bZIP gene family members, along with their transcript levels in different walnut tissues, to evaluate the transcriptional expression of this gene family during the primary culture of walnut explants and to reveal the mechanism of action of walnut bZIP genes in walnut explant browning.
The results identified 65 JrbZIP genes in the walnut genome, which were divided into 8 subfamilies and distributed on 16 chromosomes. The results of transcriptome data analysis showed that there were significant differences in the expression of four genes, namely, JrbZIP55, JrbZIP70, JrbZIP72, and JrbZIP88, under both vermiculite and agar culture conditions. There were multiple hormone (salicylic acid, abscisic acid, auxin, and gibberellin) signaling and regulatory elements that are responsive to stress (low temperature, stress, and defense) located in the promoter regions of JrbZIP55, JrbZIP70, JrbZIP72, and JrbZIP88. The walnut JrbZIP55 protein and Arabidopsis bZIP42 protein are highly homologous, and the proteins interacting with Arabidopsis bZIP42 include the AT2G19940 oxidoreductases, which act on aldehyde or oxygen-containing donors.
It is speculated that JrbZIP55 may participate in the regulation of browning in walnut explants.
碱性亮氨酸拉链（bZIP）蛋白是植物中重要的转录因子。为研究 bZIP 转录因子在核桃外植体褐变中的作用，本研究利用生物信息学软件分析了核桃 bZIP 基因家族成员及其在不同核桃组织中的转录水平，评估了该基因家族在核桃外植体初代培养过程中的转录表达情况，并揭示了核桃 bZIP 基因在核桃外植体褐变中的作用机制。
从核桃基因组中鉴定出 65 个 JrbZIP 基因，分为 8 个亚家族，分布在 16 条染色体上。转录组数据分析结果表明，在蛭石和琼脂培养条件下，有 4 个基因（JrbZIP55、JrbZIP70、JrbZIP72 和 JrbZIP88）的表达存在显著差异。JrbZIP55、JrbZIP70、JrbZIP72 和 JrbZIP88 的启动子区域存在多种激素（水杨酸、脱落酸、生长素和赤霉素）信号和调控元件，对低温、胁迫和防御等应激反应敏感。核桃 JrbZIP55 蛋白与拟南芥 bZIP42 蛋白高度同源，与拟南芥 bZIP42 相互作用的蛋白包括 AT2G19940 氧化还原酶，该酶作用于醛或含氧供体。
推测 JrbZIP55 可能参与调控核桃外植体的褐变。
