通过琥珀酸脱氢酶抑制（SDI）试验评估的各种人类肿瘤的体外化学敏感性(2)。

[In vitro chemosensitivity of various human tumors evaluated by the succinate dehydrogenase inhibition (SDI) test (2)].

作者信息

Anai H, Maehara Y, Kusumoto H, Masuda H, Miyamoto K, Fukuchi K, Tamada R, Sugimachi K

出版信息

Gan To Kagaku Ryoho. 1986 Aug;13(8):2544-8.

Abstract

In vitro chemosensitivity was evaluated by succinate dehydrogenase inhibition (SDI) test in 94 human tumors including 59 gastric cancers, 27 colo-rectal cancers and 8 malignant lymphomas. Tumor fragments were exposed to 12 kinds of antitumor drugs at ten times peak plasma concentration. Evaluable rates were 86/94 (91%) for all cases, 56/59 (95%) for gastric cancers, 22/27 (81%) for colo-rectal cancers and 8/8 (100%) for malignant lymphomas. The mean of SD activity was decreased to 48% of that of control cells with aclacinomycin, 49% with carboquone, 53% with actinomycin D, 54% with mitomycin C and 54% with daunomycin for gastric cancers, 59% with adriamycin for colo-rectal cancers and 33% with cyclophosphamide (40487 S), and 33% with actinomycin D, 37% with vinblastine and 39% with adriamycin for malignant lymphomas. When the SD activity was reduced to below 50% by antitumor drugs, the chemosensitivity was defined as positive. The antitumor drugs which had a higher chemosensitive-positive rate were aclacinomycin, carboquone and mitomycin C for gastric cancers, adriamycin for colo-rectal cancers and 40487 S, daunomycin and vinblastine for malignant lymphomas. Our results suggest that the origin of a tumor is a critical factor in its chemosensitivity.

摘要

通过琥珀酸脱氢酶抑制（SDI）试验评估了94例人类肿瘤的体外化学敏感性，其中包括59例胃癌、27例结直肠癌和8例恶性淋巴瘤。将肿瘤组织块暴露于12种抗肿瘤药物，药物浓度为血浆峰值浓度的10倍。所有病例的可评估率为86/94（91%），胃癌为56/59（95%），结直肠癌为22/27（81%），恶性淋巴瘤为8/8（100%）。对于胃癌，阿克拉霉素使SD活性平均值降至对照细胞的48%，卡波醌为49%，放线菌素D为53%，丝裂霉素C为54%，柔红霉素为54%；对于结直肠癌，阿霉素使SD活性平均值降至对照细胞的59%；对于恶性淋巴瘤，环磷酰胺（40487 S）使SD活性平均值降至对照细胞的33%，放线菌素D为33%，长春碱为37%，阿霉素为39%。当抗肿瘤药物使SD活性降至50%以下时，化学敏感性定义为阳性。化学敏感性阳性率较高的抗肿瘤药物，对于胃癌是阿克拉霉素、卡波醌和丝裂霉素C，对于结直肠癌是阿霉素，对于恶性淋巴瘤是40487 S、柔红霉素和长春碱。我们的结果表明，肿瘤起源是其化学敏感性的关键因素。