通过细胞内ATP水平评估的体外化学敏感性测试：ATP检测

[In vitro chemosensitivity testing evaluated by intracellular ATP level: ATP assay].

作者信息

Maehara Y, Anai H, Masuda H, Miyamoto K, Fukuchi K, Tamada R, Sugimachi K

出版信息

Gan To Kagaku Ryoho. 1986 Jul;13(7):2342-5.

Abstract

In vitro chemosensitivity was evaluated by bioluminescence ATP assay in 12 human tumors including 7 gastric cancers and 5 colo-rectal cancers. Tumor fragments minced with scissors were exposed to 6 kinds of antitumor drugs: carboquone, adriamycin, aclacinomycin A, mitomycin C, cisplatin and 5-FU at peak plasma concentration or ten times peak plasma concentration. After 3 days at 37 degrees C, each tumor fragment suspension was washed with phosphate-buffered saline, boiled for 3 min and assayed for its intracellular ATP level using the luciferin-luciferase method. The ATP level of the drug-untreated group was 6.01 +/- 4.55 X 10(-10) moles/mg tissue protein in gastric cancers and 9.77 +/- 8.46 X 10(-10) moles/mg tissue protein in colo-rectal cancers. The percentages of cases in which the ATP level was reduced to less than 50% by the antitumor drugs were 70% for carboquone, 11% for adriamycin, 30% for aclacinomycin A, 45% for mitomycin C, 13% for cisplatin and 18% for 5-FU at peak plasma concentration, and 90% for carboquone, 78% for adriamycin, 80% for aclacinomycin A, 82% for mitomycin C, 63% for cisplatin and 82% for 5-FU at ten times peak plasma concentration. The test for chemosensitivity prediction is thus available at peak plasma concentration of antitumor drugs in ATP assay.

摘要

通过生物发光ATP检测法对12例人类肿瘤（包括7例胃癌和5例结直肠癌）进行体外化学敏感性评估。用剪刀将肿瘤组织剪成小块，使其暴露于6种抗肿瘤药物：卡波醌、阿霉素、阿克拉霉素A、丝裂霉素C、顺铂和5-氟尿嘧啶，药物浓度为血浆峰值浓度或血浆峰值浓度的10倍。在37℃下培养3天后，将每个肿瘤组织小块悬液用磷酸盐缓冲盐水洗涤，煮沸3分钟，然后用荧光素-荧光素酶法检测其细胞内ATP水平。未用药组的ATP水平在胃癌中为6.01±4.55×10⁻¹⁰摩尔/毫克组织蛋白，在结直肠癌中为9.77±8.46×10⁻¹⁰摩尔/毫克组织蛋白。在血浆峰值浓度时，使ATP水平降低至50%以下的病例百分比分别为：卡波醌70%、阿霉素11%、阿克拉霉素A 30%、丝裂霉素C 45%、顺铂13%、5-氟尿嘧啶18%；在血浆峰值浓度的10倍时，分别为：卡波醌90%、阿霉素78%、阿克拉霉素A 80%、丝裂霉素C 82%、顺铂63%、5-氟尿嘧啶82%。因此，在ATP检测中，抗肿瘤药物血浆峰值浓度时可进行化学敏感性预测试验。