Wu Guochao, Alriksson Björn, Jönsson Leif J
Shandong Key Lab of Edible Mushroom Technology, School of Agriculture, Ludong University 264025 Yantai China.
Department of Chemistry, Umeå University SE-901 87 Umeå Sweden.
RSC Adv. 2023 Jul 4;13(29):20023-20030. doi: 10.1039/d3ra02210b. eCollection 2023 Jun 29.
By-products from hydrothermal pretreatment of lignocellulosic biomass inhibit enzymatic saccharification and microbial fermentation. Three long-chain organic extractants (Alamine 336, Aliquat 336 and Cyanex 921) were compared to two conventional organic solvents (ethyl acetate and xylene) with regard to conditioning of birch wood pretreatment liquid (BWPL) for improved fermentation and saccharification. In the fermentation experiments, extraction with Cyanex 921 resulted in the best ethanol yield, 0.34 ± 0.02 g g on initial fermentable sugars. Extraction with xylene also resulted in a relatively high yield, 0.29 ± 0.02 g g, while cultures consisting of untreated BWPL and BWPL treated with the other extractants exhibited no ethanol formation. Aliquat 336 was most efficient with regard to removing by-products, but the residual Aliquat after the extraction was toxic to yeast cells. Enzymatic digestibility increased by 19-33% after extraction with the long-chain organic extractants. The investigation demonstrates that conditioning with long-chain organic extractants has the potential to relieve inhibition of both enzymes and microbes.
木质纤维素生物质水热预处理产生的副产物会抑制酶解糖化和微生物发酵。将三种长链有机萃取剂（阿拉明336、季铵盐336和Cyanex 921）与两种传统有机溶剂（乙酸乙酯和二甲苯）进行比较，以研究对桦木预处理液（BWPL）进行预处理，从而改善发酵和糖化效果。在发酵实验中，用Cyanex 921萃取后乙醇产量最高，基于初始可发酵糖计算为0.34±0.02 g/g。用二甲苯萃取也能得到相对较高的产量，为0.29±0.02 g/g，而由未处理的BWPL和用其他萃取剂处理过的BWPL组成的培养物未产生乙醇。季铵盐336在去除副产物方面效率最高，但萃取后残留的季铵盐对酵母细胞有毒性。用长链有机萃取剂萃取后，酶解消化率提高了19% - 33%。该研究表明，用长链有机萃取剂进行预处理有缓解酶和微生物抑制作用的潜力。
