使用双水相体系轻松纯化经 DNA 修饰的小颗粒和软材料。

A facile method for purifying DNA-modified small particles and soft materials using aqueous two-phase systems.

机构信息

Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, P. R. China.

出版信息

Chem Commun (Camb). 2023 Jul 20;59(59):9130-9133. doi: 10.1039/d3cc02350h.

DOI:10.1039/d3cc02350h

PMID:37409364

Abstract

We herein report a convenient and effective method for the purification of DNA-conjugated materials with a benchtop minicentrifuge. We demonstrate the fast isolation of DNA-modified small gold nanoparticles (5 nm), liposomes, and DNA nanostructures using fluorescent methods and gel electrophoresis. Our method is cost-effective and efficient and would accelerate the development of DNA nanotechnology.

摘要

我们在此报告了一种使用台式迷你离心机纯化 DNA 偶联材料的便捷有效方法。我们使用荧光法和凝胶电泳证明了快速分离 DNA 修饰的小金纳米颗粒（5nm）、脂质体和 DNA 纳米结构。我们的方法具有成本效益和高效率，将加速 DNA 纳米技术的发展。