Wang Huan, Yang Xiaoshan, Liu Jin, Xu Jingwei, Zhang Ruimin, Zheng Junnan, Shen Bo, Sun Yan, Zhou Dan
Department of Pathogen Biology, Nanjing Medical University, Nanjing, China.
Pest Manag Sci. 2023 Nov;79(11):4463-4473. doi: 10.1002/ps.7648. Epub 2023 Jul 21.
Current mosquito-borne disease vector control strategies, largely based on chemical insecticides, are seriously threatened by increasing resistance worldwide. There is also growing concerned about the adverse effects of insecticides on nontarget organisms and the environment, therefore effective and ecologically friendly alternative approaches are urgently needed. Targeting critical steps of reproduction is considered a potential way to control mosquito populations. Herein, we focused on the roles of chitin synthase A (encoded by chsa) in the reproduction of female mosquitoes.
The injection of small interfering RNA targeting Cpchsa in female Culex pipiens pallens (Diptera: Culicidae) had antireproductive effects, including decreased follicle numbers, egg-laying, and hatching rate. Scanning electron microscopy observations showed that Cpchsa silencing caused a defective egg envelope, including absence of the vitelline membrane and cracked chorion layers, which resulted in abnormal permeability. Widely distributed nurse cell apoptosis and follicular epithelial cell autophagy were observed in Cpchsa-silenced ovaries during the vitellogenesis phase. Consistent with the detective egg envelope formation during oogenesis, the exochorionic eggshell structures were also affected in eggs deposited by Cpchsa-silenced mosquitoes.
This study provided fundamental evidence for the role of chitin synthase A in the female reproductive process of mosquitoes and might result in a novel alternative strategy for mosquito control. © 2023 Society of Chemical Industry.
目前主要基于化学杀虫剂的蚊媒疾病病媒控制策略，正受到全球范围内日益增加的抗药性的严重威胁。人们也越来越关注杀虫剂对非靶标生物和环境的不利影响，因此迫切需要有效且生态友好的替代方法。针对生殖关键步骤被认为是控制蚊虫种群的一种潜在途径。在此，我们聚焦于几丁质合酶A（由chsa编码）在雌蚊生殖中的作用。
向淡色库蚊（双翅目：蚊科）雌蚊注射靶向Cpchsa的小干扰RNA具有抗生殖作用，包括卵泡数量减少、产卵量和孵化率降低。扫描电子显微镜观察表明，Cpchsa基因沉默导致卵包膜缺陷，包括卵黄膜缺失和卵壳层破裂，从而导致通透性异常。在卵黄发生期，在Cpchsa基因沉默的卵巢中观察到广泛分布的滋养细胞凋亡和卵泡上皮细胞自噬。与卵子发生过程中卵包膜形成缺陷一致，Cpchsa基因沉默的蚊子所产卵子的卵壳外层结构也受到影响。
本研究为几丁质合酶A在蚊子雌性生殖过程中的作用提供了基础证据，并可能产生一种新的蚊虫控制替代策略。© 2023化学工业协会。
