Jilin Provincial Key Laboratory of Nutrition and Functional Food, Jilin University, Changchun, 130062, People's Republic of China.
College of Food Science and Engineering, Jilin University, Changchun, 130062, People's Republic of China.
Food Funct. 2023 Jul 17;14(14):6718-6729. doi: 10.1039/d3fo01810e.
The Maillard reaction (MR) is inevitable in food processing and daily cooking, but whether the MR degree would affect the biological activity of the protein remains unknown. In this study, we used untargeted metabolomics techniques to explore the effects of two different levels of Maillard reaction products (MRPs) of ovalbumin (OVA) on metabolites in colitis mice. Studies have shown that MR could affect protein metabolites and MRPs of OVA could reduce the concentrations of IL-6 and IL-1β and intestinal permeability. Metabolomics results showed that the degree of MR affected the abundance of oligopeptides and bile acids . This study revealed that MRPs could regulate the abundance of metabolites such as taurocholic acid and putrescine, and repair the intestinal barrier in colitis mice through signaling pathways such as secondary bile acid biosynthesis, bile secretion and ABC transporters. The investigation has significant implications for the digestion properties and metabolite regulation of MRPs , and also promotes the application of MRPs in functional foods.
美拉德反应（MR）在食品加工和日常烹饪中不可避免，但 MR 程度是否会影响蛋白质的生物活性尚不清楚。在这项研究中，我们使用非靶向代谢组学技术来探索两种不同水平的卵清蛋白（OVA）美拉德反应产物（MRP）对结肠炎小鼠代谢物的影响。研究表明，MR 可以影响蛋白质代谢物，OVA 的 MRP 可以降低 IL-6 和 IL-1β 的浓度和肠通透性。代谢组学结果表明，MR 的程度影响寡肽和胆汁酸的丰度。这项研究表明，MRP 可以通过次级胆汁酸生物合成、胆汁分泌和 ABC 转运体等信号通路，调节牛磺胆酸和腐胺等代谢物的丰度，修复结肠炎小鼠的肠道屏障。该研究对 MRP 的消化特性和代谢物调节具有重要意义，并促进了 MRP 在功能性食品中的应用。
