Institute for Information and Communication Technologies, Electronics and Applied Mathematics, UCLouvain, 1348 Louvain-la-Neuve, Belgium.
Laboratory of Food and Environmental Microbiology, Earth and Life Institute, UCLouvain, 1348 Louvain-la-Neuve, Belgium.
ACS Sens. 2023 Jul 28;8(7):2627-2634. doi: 10.1021/acssensors.3c00467. Epub 2023 Jul 6.
In this work, we developed a biosensor for the indirect detection of bacteria via their lysate. The developed sensor is based on porous silicon membranes, which are known for their many attractive optical and physical properties. Unlike traditional porous silicon biosensors, the selectivity of the bioassay presented in this work does not rely on bio-probes attached to the sensor surface; the selectivity is added to the analyte itself, by the addition of lytic enzymes that target only the desired bacteria. The resulting bacterial lysate is then able to penetrate into the porous silicon membrane and affects its optical properties, while intact bacteria accumulate on top of the sensor. The porous silicon sensors, fabricated using standard microfabrication techniques, are coated with TiO layers using atomic layer deposition. These layers serve as passivation but also enhance the optical properties. The performance of the TiO-coated biosensor is tested for the detection of , using the bacteriophage-encoded PlyB221 endolysin as the lytic agent. The sensitivity of the biosensor is much improved compared to previous works, reaching 10 CFU/mL, with a total assay time of 1 h 30 min. The selectivity and versatility of the detection platform are also demonstrated, as is the detection of in a complex analyte.
在这项工作中，我们开发了一种基于多孔硅膜的生物传感器，用于间接检测细菌的裂解物。多孔硅膜具有许多吸引人的光学和物理特性，这是众所周知的。与传统的多孔硅生物传感器不同，本工作中提出的生物测定法的选择性不依赖于附着在传感器表面的生物探针; 选择性是通过添加仅针对所需细菌的裂解酶来添加到分析物本身的。然后，所得细菌裂解物能够穿透多孔硅膜并影响其光学性质，而完整的细菌则积聚在传感器顶部。使用标准微制造技术制造的多孔硅传感器使用原子层沉积技术涂覆 TiO 层。这些层不仅起到钝化作用，而且还增强了光学性能。使用噬菌体编码的 PlyB221 内溶素作为裂解剂，测试了 TiO 涂层生物传感器检测 的性能。与以前的工作相比，该生物传感器的灵敏度大大提高，达到 10 CFU/mL，总检测时间为 1 小时 30 分钟。还证明了检测平台的选择性和多功能性，以及在复杂分析物中检测 的能力。
