Jenie S N Aisyiyah, Plush Sally E, Voelcker Nicolas H
ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Future Industries Institute, University of South Australia, Mawson Lakes, SA, 5095, Australia.
Research Centre for Chemistry, Indonesian Institute of Sciences, PUSPIPTEK, Serpong, Tangerang, Banten, 15314, Indonesia.
Pharm Res. 2016 Oct;33(10):2314-36. doi: 10.1007/s11095-016-1889-1. Epub 2016 Feb 25.
Luminescence-based detection paradigms have key advantages over other optical platforms such as absorbance, reflectance or interferometric based detection. However, autofluorescence, low quantum yield and lack of photostability of the fluorophore or emitting molecule are still performance-limiting factors. Recent research has shown the need for enhanced luminescence-based detection to overcome these drawbacks while at the same time improving the sensitivity, selectivity and reducing the detection limits of optical sensors and biosensors. Nanostructures have been reported to significantly improve the spectral properties of the emitting molecules. These structures offer unique electrical, optic and magnetic properties which may be used to tailor the surrounding electrical field of the emitter. Here, the main principles behind luminescence and luminescence enhancement-based detections are reviewed, with an emphasis on europium complexes as the emitting molecule. An overview of the optical porous silicon microcavity (pSiMC) as a biosensing platform and recent proof-of-concept examples on enhanced luminescence-based detection using pSiMCs are provided and discussed.
基于发光的检测范式相对于其他光学平台（如基于吸光度、反射率或干涉测量的检测）具有关键优势。然而，自发荧光、低量子产率以及荧光团或发射分子缺乏光稳定性仍是限制性能的因素。最近的研究表明，需要增强基于发光的检测以克服这些缺点，同时提高光学传感器和生物传感器的灵敏度、选择性并降低检测限。据报道，纳米结构可显著改善发射分子的光谱特性。这些结构具有独特的电学、光学和磁学性质，可用于调整发射体周围的电场。在此，回顾了基于发光和发光增强检测背后的主要原理，重点是铕配合物作为发射分子。提供并讨论了作为生物传感平台的光学多孔硅微腔（pSiMC）的概述以及使用pSiMC进行基于发光增强检测的最新概念验证示例。
