Clark B L, Emery D L, Stewart D J, Dufty J H, Anderson D A
Aust Vet J. 1986 Apr;63(4):107-10. doi: 10.1111/j.1751-0813.1986.tb07675.x.
Calves were immunised with fractions of Fusobacterium necrophorum incorporated in mineral oil adjuvant and then each foot was experimentally exposed to interdigital necrobacillosis (foot abscess) by subcutaneous injection of homologous organisms through the interdigital skin. The number of cells from an 18 h liquid culture that might be expected to cause 50% of the feet of control calves to develop marked swellings following subcutaneous injection was shown to be approximately 2.2 X 10(8) cells. Immunity was shown to be associated with antigens that were located in the supernatant of the culture, and which may be identical with or closely associated with the exotoxins.
用掺入矿物油佐剂的坏死梭杆菌组分对犊牛进行免疫，然后通过在趾间皮肤下皮下注射同源微生物，使每只脚实验性感染趾间坏死杆菌病（足部脓肿）。结果显示，皮下注射后，预计会使50%的对照犊牛足部出现明显肿胀的18小时液体培养物中的细胞数量约为2.2×10⁸个细胞。免疫与位于培养物上清液中的抗原相关，这些抗原可能与外毒素相同或密切相关。
