Corner L A, Collins N D, Vaughan J A
CSIRO, Animal Health Research Laboratory, Vic, Australia.
Vet Microbiol. 1996 Jan;48(1-2):1-7. doi: 10.1016/0378-1135(95)00142-5.
An experimental procedure is described for the production of foot abscess in sheep that mimics the natural disease. Lesions were produced by the intradermal inoculation of suspensions of Fusobacterium necrophorum biotype AB containing from 5 x 10(2) to 5 x 10(8) bacteria, into interdigital skin devitalized by freezing with liquid nitrogen. A dose of 5 x 10(5) bacteria induced the development of foot abscess in 3 of 4 and 8 of 8 inoculated feet. It was found that to produce foot abscess in devitalized tissue required between 10(3) and 10(6) fewer bacteria than were necessary to produce similar lesions in healthy tissue.
描述了一种在绵羊身上制造足部脓肿的实验方法，该方法模拟自然疾病。通过将含有5×10²至5×10⁸个细菌的坏死梭杆菌生物型AB悬浮液皮内接种到经液氮冷冻而失活的趾间皮肤中，产生病变。5×10⁵个细菌的剂量在4只接种足部中的3只以及8只接种足部中的8只引发了足部脓肿。结果发现，在失活组织中产生足部脓肿所需的细菌比在健康组织中产生类似病变所需的细菌少10³至10⁶个。
