Tognoli L, Colombo R
Biochem J. 1986 Apr 1;235(1):45-8. doi: 10.1042/bj2350045.
Fusicoccin (FC), a natural diterpene glucoside able to stimulate electrogenic H+ extrusion in higher plants, has been shown to stimulate the phosphorylation of a polypeptide of molecular mass approx. 33 kDa in intact cultured cells of sycamore (Acer pseudoplatanus). The effect is specific, rapid and insensitive to cycloheximide. The presence of the 33 kDa polypeptide and the stimulation by FC have been observed in SDS-containing cell homogenates and in the microsomal and soluble fractions after cell fractionation.
壳梭孢菌素(FC)是一种能够刺激高等植物中质子的主动外排的天然二萜糖苷,已证明它能刺激大约33 kDa的多肽在悬铃木(Acer pseudoplatanus)完整培养细胞中的磷酸化。这种作用具有特异性、快速性,且对放线菌酮不敏感。在含有十二烷基硫酸钠的细胞匀浆中以及细胞分级分离后的微粒体和可溶部分中都观察到了33 kDa多肽的存在以及壳梭孢菌素对其的刺激作用。