蔗糖饥饿对梧桐（Acer pseudoplatanus）细胞碳水化合物和无机磷状态的影响。
Effect of sucrose starvation on sycamore (Acer pseudoplatanus) cell carbohydrate and Pi status.
作者信息
Rébeillé F, Bligny R, Martin J B, Douce R
出版信息
Biochem J. 1985 Mar 15;226(3):679-84. doi: 10.1042/bj2260679.
Abstract
The mobilization of stored carbohydrates during sucrose starvation was studied with sycamore (Acer pseudoplatanus) cells. When sucrose was omitted from the nutrient medium, the intracellular sucrose pool decreased rapidly during the first hours of the experiment, whereas the starch content remained practically unchanged. After 10h of sucrose starvation, starch hydrolysis replaced sucrose breakdown. From this moment, the phosphate-ester pool and respiration rate decreased with time. Conversely, the intracellular Pi concentration increased. 31P n.m.r. of intact sycamore cells indicated that, under these conditions, most of the Pi accumulated in the vacuole. These results strongly suggest that starch breakdown, in contrast with sucrose hydrolysis, is not rapid enough to maintain a high cellular metabolism.
摘要
利用悬铃木（Acer pseudoplatanus）细胞研究了蔗糖饥饿期间储存碳水化合物的动员情况。当从营养培养基中去除蔗糖时，在实验的最初几个小时内，细胞内蔗糖库迅速减少，而淀粉含量实际上保持不变。蔗糖饥饿10小时后，淀粉水解取代了蔗糖分解。从这一刻起，磷酸酯库和呼吸速率随时间下降。相反，细胞内无机磷（Pi）浓度增加。完整悬铃木细胞的31P核磁共振表明，在这些条件下，大部分Pi积累在液泡中。这些结果有力地表明，与蔗糖水解相反，淀粉分解的速度不足以维持高细胞代谢。
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