College of Life Sciences and Bioengineering, School of Physical Science and Engineering, Beijing Jiaotong University, Beijing 100044, PR China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Shenzhen Key Laboratory of Agricultural Synthetic Biology, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, PR China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Sciences, South China Agricultural University, Guangzhou 510642, PR China.
College of Life Sciences and Bioengineering, School of Physical Science and Engineering, Beijing Jiaotong University, Beijing 100044, PR China.
Ecotoxicol Environ Saf. 2023 Sep 15;263:115214. doi: 10.1016/j.ecoenv.2023.115214. Epub 2023 Jul 5.
Brassica campestris L., a cadmium (Cd) hyperaccumulating herbaceous plant, is considered as a promising candidate for the bioremediation of Cd pollution. However, the molecular mechanisms regulating these processes remain unclear. The present work, using proteome studies combined with a transcriptome analysis, was carried out to reveal the response mechanisms of the hairy roots of Brassica campestris L. under Cd stress. Significant tissue necrosis and cellular damage occurred, and Cd accumulation was observed in the cell walls and vacuoles of the hairy roots. Through quantitative proteomic profiling, a total of 1424 differentially expressed proteins (DEPs) were identified, and are known to be enriched in processes including phenylalanine metabolism, plant hormone signal transduction, cysteine and methionine metabolism, protein export, isoquinoline alkaloid biosynthesis and flavone biosynthesis. Further studies combined with a transcriptome analysis found that 118 differentially expressed genes (DEGs) and their corresponding proteins were simultaneously up- or downregulated. Further Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis of the 118 shared DEGs and DEPs indicated their involvement in calcium, ROS and hormone signaling-mediated response, including regulation of carbohydrate and energy metabolism, biosynthesis of GSH, PCs and phenylpropanoid compounds that play vital roles in the Cd tolerance of Brassica campestris L. Our findings contribute to a better understanding of the regulatory networks of Brassica campestris L. under Cd stress, as well as provide valuable information on candidate genes (e.g., BrPAL, BrTAT, Br4CL, BrCDPK, BrRBOH, BrCALM, BrABCG1/2, BrVIP, BrGCLC, BrilvE, BrGST12/13/25). These results are of particular importance to the subsequent development of promising transgenic plants that will hyperaccumulate heavy metals and efficient phytoremediation processes.
芥菜（Brassica campestris L.）是一种镉（Cd）超积累草本植物，被认为是修复 Cd 污染的有前途的候选植物。然而，调节这些过程的分子机制尚不清楚。本研究采用蛋白质组学研究与转录组分析相结合的方法，揭示了 Cd 胁迫下芥菜毛状根的响应机制。毛状根组织发生明显坏死和细胞损伤，Cd 积累在细胞壁和液泡中。通过定量蛋白质组学分析，共鉴定到 1424 个差异表达蛋白（DEPs），这些蛋白主要富集在苯丙氨酸代谢、植物激素信号转导、半胱氨酸和蛋氨酸代谢、蛋白质输出、异喹啉生物碱生物合成和黄酮类生物合成等过程中。进一步的研究结合转录组分析发现，118 个差异表达基因（DEGs）及其相应的蛋白同时上调或下调。对这 118 个共有 DEGs 和 DEPs 的基因本体论和京都基因与基因组百科全书分析表明，它们参与了钙、ROS 和激素信号转导介导的响应，包括碳水化合物和能量代谢的调节、GSH、PCs 和苯丙烷类化合物的生物合成，这些化合物在芥菜 Cd 耐受性中发挥着重要作用。我们的研究结果有助于更好地理解芥菜在 Cd 胁迫下的调控网络，并为候选基因（如 BrPAL、BrTAT、Br4CL、BrCDPK、BrRBOH、BrCALM、BrABCG1/2、BrVIP、BrGCLC、BrilvE、BrGST12/13/25）的研究提供有价值的信息。这些结果对于随后开发能够超积累重金属和进行有效植物修复的转基因植物具有重要意义。
