Jimenez S A, Rao V H, Reginato A M, Yankowski R
Biochem Biophys Res Commun. 1986 Jul 31;138(2):835-41. doi: 10.1016/s0006-291x(86)80572-1.
Chondrocytes from the presumptive calcification region of 20 day old embryonic chick sternum were found to synthesize a 70 Kd Type X procollagen precursor in addition to the previously described 59 Kd Type X collagen molecules. The 70 Kd molecules exhibited an additional cyanogen bromide peptide, contained a disulfide-bonded domain, and were converted into the 59 Kd moieties during pulse-chase experiments. The conversion of the 70 Kd to the 59 Kd Type X collagen was prevented upon microtubular transport inhibition with colchicine and resulted in tissue accumulation of the 70 Kd Type X procollagen.
研究发现，20日龄鸡胚胸骨假定钙化区域的软骨细胞除了能合成先前描述的59千道尔顿X型胶原蛋白分子外，还能合成一种70千道尔顿X型前胶原前体。70千道尔顿的分子表现出一个额外的溴化氰肽，含有一个二硫键结合结构域，并且在脉冲追踪实验中会转化为59千道尔顿的部分。用秋水仙碱抑制微管运输后，70千道尔顿的X型胶原蛋白向59千道尔顿的转化受到阻碍，并导致70千道尔顿X型前胶原在组织中积累。
