来自韩国的一只金球蛛（螯肢亚门，蛛形纲）的完整线粒体基因组。
Complete mitochondrial genome of a golden orb-web spider (Chelicerata, Arachnida) from South Korea.
作者信息
Choi Eun Hwa, Hwang Ui Wook
机构信息
Department of Biology Education, Teachers College and Institute for Phylogenomics and Evolution, Kyungpook National University, Daegu, South Korea.
Institute for Korean Herb-Bio Convergence Promotion, Kyungpook National University, Daegu, South Korea.
出版信息
Mitochondrial DNA B Resour. 2023 Jul 4;8(7):723-725. doi: 10.1080/23802359.2021.1955633. eCollection 2023.
The mitochondrial genome of a golden orb-web spider (L. Koch, 1878) from South Korea is determined and characterized in detail, which is the second mitochondrial genome reported from this species: the first was published from the Chinese sample by Pan et al. (2016). It was 14,436 bp in length being composed of 13 protein-coding genes (PCGs), 22 transfer RNA genes, two ribosomal RNA genes, and one control region (CR). It has a base composition of 35.99% for 'A,' 14.88% for 'G,' 9.09% for 'C,' and 40.04% for 'T.' Comparing the South Korean and Chinese mitochondrial genomes, we observed 8% nucleotide sequence differences between their CRs, caused by the different numbers and sorts of possessed tandem repeats, suggesting a promising molecular marker to distinguish South Korean individuals from Chinese ones. The phylogenetic trees using the maximum likelihood (ML) method were reconstructed with nucleotides (without 3rd codon position) and amino acids from 13 PCGs, respectively, which consistently confirmed that (Subfamily Nephilinae) from South Korea and China are clustered together, distinctly separated from the other subfamily Araneinae in the monophyletic family Araneidae.
对一只来自韩国的金球织网蜘蛛（L. Koch，1878）的线粒体基因组进行了详细测定和特征分析，这是该物种报道的第二个线粒体基因组：第一个是潘等人（2016年）发表的来自中国样本的线粒体基因组。它全长14436 bp，由13个蛋白质编码基因（PCGs）、22个转运RNA基因、两个核糖体RNA基因和一个控制区（CR）组成。其碱基组成为：A占35.99%，G占14.88%，C占9.09%，T占40.04%。比较韩国和中国的线粒体基因组，我们发现它们的控制区之间存在8%的核苷酸序列差异，这是由所拥有的串联重复序列的数量和种类不同引起的，这表明它是区分韩国个体和中国个体的一个有前景的分子标记。分别使用最大似然（ML）方法，用13个PCGs的核苷酸（不包括第三密码子位置）和氨基酸重建了系统发育树，结果一致证实来自韩国和中国的（络新妇科）聚集在一起，与单系科园蛛科中的另一个亚科园蛛亚科明显分开。
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