Department of Biological Sciences and Biotechnology, Chungbuk National University, Cheongju, 28644, Republic of Korea.
Division of Forest Biodiversity, Korea National Arboretum, Pocheon, 11186, Republic of Korea.
Genes Genomics. 2023 Jun;45(6):827-833. doi: 10.1007/s13258-023-01378-6. Epub 2023 Apr 4.
The endangered longhorn beetle Callipogon (Eoxenus) relictus, which was designated as a natural monument since 1968 in Korea is still attracting public concern because of its extraordinary body size. Although mitochondrial genome data of this species was reported using Korean individual in 2017, start codon of cox1 is controversial and the secondary structures of transfer RNAs have not been illustrated.
To report complete mitochondrial genome of Callipogon (Eoxenus) relictus from Chinese breed.
We used dissected muscle tissues from an adult of Callipogon (Eoxenus) relictus. A total of 19,276,266,645 bp from 127,657,395 reads were generated. The raw reads were assembled to mitochondrial genome data and annotated. Folded structures of transfer RNAs were drawn. Phylogenetic relationships were estimated by maximum likelihood and Bayesian inference analyses.
The mitochondrial genome of C. relictus was 15,745 bp in length and composed of 37 genes including 13 protein-coding genes (PCGs), two ribosomal RNAs, and 22 transfer RNAs. The overall base composition was 38.40% for A, 30.98% for T, 11.06% for G, and 19.56% for C. Most transfer RNAs were folded into the typical clover-leaf structure except trnS1. Phylogenetic analyses confirmed the monophyletic status of each subfamily.
Mitochondrial genome composition was consistent with previous research, however, we suggest another start codon of cox1 gene and provide illustrated secondary structures of transfer RNAs. Phylogenetic analyses showed that subfamilies Cerambycinae and Prioninae are closely related.
自 1968 年以来，韩国一直将濒危长角甲虫 Callipogon (Eoxenus) relictus 指定为自然纪念碑，因其体型异常而仍然引起公众关注。尽管 2017 年曾使用韩国个体报道了该物种的线粒体基因组数据，但 cox1 的起始密码子存在争议，并且转移 RNA 的二级结构尚未说明。
报告来自中国品种的 Callipogon (Eoxenus) relictus 的完整线粒体基因组。
我们使用成年 Callipogon (Eoxenus) relictus 的解剖肌肉组织。从 127,657,395 条读取中生成了总共 19,276,266,645 bp 的数据。原始读取被组装成线粒体基因组数据并进行注释。绘制转移 RNA 的折叠结构。通过最大似然和贝叶斯推断分析估计系统发育关系。
C. relictus 的线粒体基因组长 15,745 bp，由 37 个基因组成，包括 13 个蛋白质编码基因（PCGs）、两个核糖体 RNA 和 22 个转移 RNA。总碱基组成分别为 A 为 38.40%，T 为 30.98%，G 为 11.06%，C 为 19.56%。大多数转移 RNA 折叠成典型的三叶形结构，除了 trnS1 之外。系统发育分析证实了每个亚科的单系性。
线粒体基因组组成与先前的研究一致，但我们建议 cox1 基因的另一个起始密码子，并提供转移 RNA 的说明性二级结构。系统发育分析表明，Cerambycinae 和 Prioninae 亚科密切相关。
