Stomatological Center of Peking University Shenzhen Hospital, Guangdong Provincial High-level Clinical Key Specialty, Guangdong Province Engineering Research Center of Oral Disease Diagnosis and Treatment, Shenzhen, China.
Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China.
Tissue Eng Part C Methods. 2023 Jul;29(7):321-331. doi: 10.1089/ten.TEC.2023.0041.
Mesenchymal stem cell and 3D printing-based bone tissue engineering present a promising technique to repair large-volume bone defects. Its success is highly dependent on cell attachment, spreading, osteogenic differentiation, and survival of stem cells on 3D-printed scaffolds. In this study, we applied human salivary histatin-1 (Hst1) to enhance the interactions of human adipose-derived stem cells (hASCs) on 3D-printed β-tricalcium phosphate (β-TCP) bioceramic scaffolds. Fluorescent images showed that Hst1 significantly enhanced the adhesion of hASCs to both bioinert glass and 3D-printed β-TCP scaffold. In addition, Hst1 was associated with significantly higher proliferation and osteogenic differentiation of hASCs on 3D-printed β-TCP scaffolds. Moreover, coating 3D-printed β-TCP scaffolds with histatin significantly promotes the survival of hASCs . The ERK and p38 but not JNK signaling was found to be involved in the superior adhesion of hASCs to β-TCP scaffolds with the aid of Hst1. In conclusion, Hst1 could significantly promote the adhesion, spreading, osteogenic differentiation, and survival of hASCs on 3D-printed β-TCP scaffolds, bearing a promising application in stem cell/3D printing-based constructs for bone tissue engineering.
基于间充质干细胞和 3D 打印的骨组织工程技术为修复大体积骨缺损提供了一种很有前途的方法。其成功高度依赖于干细胞在 3D 打印支架上的黏附、铺展、成骨分化和存活。在这项研究中，我们应用人唾液组蛋白-1（Hst1）来增强人脂肪源性干细胞（hASCs）与 3D 打印β-磷酸三钙（β-TCP）生物陶瓷支架的相互作用。荧光图像显示，Hst1 显著增强了 hASCs 与生物惰性玻璃和 3D 打印β-TCP 支架的黏附。此外，Hst1 与 hASCs 在 3D 打印β-TCP 支架上的增殖和成骨分化显著相关。此外，在 3D 打印β-TCP 支架上涂覆组蛋白显著促进了 hASCs 的存活。研究发现，ERK 和 p38 而不是 JNK 信号通路参与了 Hst1 辅助下 hASCs 对β-TCP 支架更好的黏附。总之，Hst1 可显著促进 hASCs 在 3D 打印β-TCP 支架上的黏附、铺展、成骨分化和存活，有望在基于干细胞/3D 打印的骨组织工程构建物中得到应用。
