Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine. No. 639 Zhizaoju Road, Shanghai 200001, China.
Department of Radiology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine. No. 280 Mohe Road, Shanghai 201999, China.
J Mater Chem B. 2023 Feb 22;11(8):1725-1738. doi: 10.1039/d2tb02280j.
Cellular bioactivity and tissue regeneration can be affected by coatings on tissue-engineered scaffolds. Using mussel-inspired polydopamine (PDA) is a convenient and effective approach to surface modification. Therefore, 3D-printed β-tricalcium phosphate (β-TCP) scaffolds were coated with PDA in this study. The effects of the scaffolds on the adhesion and osteogenic differentiation of seeded bone marrow mesenchymal stem cells (BMSCs) and on new-bone formation were investigated. The potential mechanisms and related differential genes were assessed using mRNA sequencing. It was seen that PDA coating increased the surface roughness of the 3D-printed β-TCP scaffolds. Furthermore, it prompted the adhesion and osteogenic differentiation of seeded BMSCs. mRNA sequencing analysis revealed that PDA coating might affect the osteogenic differentiation of BMSCs through the calcium signaling pathway, Wnt signaling pathway, TGF-beta signaling pathway, Moreover, the expression of osteogenesis-related genes, such as R-spondin 1 and chemokine c-c-motif ligand 2, was increased. Finally, both the 3D-printed β-TCP scaffolds and PDA-coated scaffolds could significantly accelerate the formation of new bone in critical-size calvarial defects in rats compared with the control group; and the new bone formation was obviously higher in the PDA-coated scaffolds than in β-TCP scaffolds. In summary, 3D-printed β-TCP scaffolds with a PDA coating can improve the physicochemical characteristics and cellular bioactivity of the scaffold surface for bone regeneration. Potential differential genes were identified, which can be used as a foundation for further research.
细胞生物活性和组织再生会受到组织工程支架涂层的影响。使用贻贝启发的聚多巴胺（PDA）是一种方便有效的表面改性方法。因此，本研究在 3D 打印的β-磷酸三钙（β-TCP）支架上涂覆了 PDA。研究了支架对种子骨髓间充质干细胞（BMSCs）黏附和成骨分化以及新骨形成的影响。使用 mRNA 测序评估了潜在机制和相关差异基因。结果表明，PDA 涂层增加了 3D 打印的β-TCP 支架的表面粗糙度。此外，它促进了种子 BMSCs 的黏附和成骨分化。mRNA 测序分析表明，PDA 涂层可能通过钙信号通路、Wnt 信号通路、TGF-β 信号通路影响 BMSCs 的成骨分化。此外，成骨相关基因如 R-spondin 1 和趋化因子 C-C 基序配体 2 的表达增加。最后，与对照组相比，3D 打印的β-TCP 支架和 PDA 涂层支架都能显著加速大鼠临界尺寸颅骨缺损中新骨的形成，并且 PDA 涂层支架的新骨形成明显高于β-TCP 支架。综上所述，具有 PDA 涂层的 3D 打印β-TCP 支架可以改善支架表面的理化特性和细胞生物活性，促进骨再生。鉴定出了潜在的差异基因，可以作为进一步研究的基础。
