Niemann H, Elsaesser F
Biol Reprod. 1986 Aug;35(1):10-6. doi: 10.1095/biolreprod35.1.10.
The physiological significance of estradiol-17beta for the early embryonic development in the pig was investigated in vitro by four different experimental designs. A total of 1635 morphologically intact morulae were cultured in vitro in Krebs-Ringer bicarbonate solution supplemented with 10% heat-inactivated lamb serum, and the blastocyst formation rate (BFR) was recorded after 24 or 48 h. The addition of estradiol-17 beta (0.1 nM, 1 nM, 100 nM), progesterone (100 nM, 500 nM) or cortisol (100 nM) to the culture medium did not affect BFR (95 to 100%, Experiment 1). Similarly, adding charcoal-stripped lamb serum to the medium instead of normal lamb serum in the absence or presence of 1 nM estradiol-17 beta had no effect (93 to 95% BFR, Experiment 2). The antiestrogen Nafoxidine, however, at a concentration of 15 micrograms/ml, significantly (p less than 0.01) reduced BFR to 13.3 +/- 5.8% compared to controls (93.3 +/- 4.2%, Experiment 3). Supplementation with estradiol-17 beta (1 nM) in the presence of 15 micrograms/ml Nafoxidine significantly (p less than 0.01) improved BFR to 57.2 +/- 8.9%. Higher concentrations of estradiol-17 beta (100 nM, 100 microM) did not further enhance BFR. The stimulatory effects of estradiol-17 beta were specific since the BFR remained low in the presence of 100 nM progesterone (10.0 +/- 4.5%) or 100 nM cortisol (3.3 +/- 3.3%). Addition of 5% estradiol-17 beta-antiserum to the culture medium (Experiment 4) significantly (p less than 0.01) reduced BRF to 51.9 +/- 6.7% compared to controls (93.1 +/- 2.2%).(ABSTRACT TRUNCATED AT 250 WORDS)
通过四种不同的实验设计在体外研究了17β-雌二醇对猪早期胚胎发育的生理意义。总共1635个形态完整的桑葚胚在补充有10%热灭活羔羊血清的 Krebs-Ringer 碳酸氢盐溶液中进行体外培养，并在24或48小时后记录囊胚形成率（BFR）。向培养基中添加17β-雌二醇（0.1 nM、1 nM、100 nM）、孕酮（100 nM、500 nM）或皮质醇（100 nM）不影响BFR（95%至100%，实验1）。同样，在不存在或存在1 nM 17β-雌二醇的情况下，用活性炭处理过的羔羊血清代替正常羔羊血清添加到培养基中没有影响（BFR为93%至95%，实验2）。然而，抗雌激素萘氧啶浓度为15微克/毫升时，与对照组相比（93.3%±4.2%，实验3），显著（p<0.01）降低BFR至13.3%±5.8%。在存在15微克/毫升萘氧啶的情况下补充17β-雌二醇（1 nM）显著（p<0.01）将BFR提高到57.2%±8.9%。更高浓度的17β-雌二醇（100 nM、100 microM）没有进一步提高BFR。17β-雌二醇的刺激作用是特异性的，因为在存在100 nM孕酮（10.0%±4.5%）或100 nM皮质醇（3.3%±3.3%）时BFR仍然很低。向培养基中添加5%的17β-雌二醇抗血清（实验4）与对照组相比（93.1%±2.2%）显著（p<0.01）降低BRF至51.9%±6.7%。（摘要截断于250字）
