刚地弓形虫：速殖子在乳过氧化物酶介导的放射性碘化过程中内在过氧化物酶活性的证明
Toxoplasma gondii: demonstration of intrinsic peroxidase activity during lacto-peroxidase mediated radioiodination of tachyzoites.
作者信息
Gallois Y, Tricaud A, Foussard F, Dubremetz J F, Hodbert J, Girault A, Mauras G
出版信息
Biomed Pharmacother. 1986;40(3):111-3.
Tachyzoites of Toxoplasma gondii have been radioiodinated under various conditions with or without lactoperoxidase, with glucose oxidase being used to generate hydrogen peroxide. Erythrocytes were iodinated simultaneously as a control. In our conditions, tachyzoites were more intensely labelled in the absence of lactoperoxidase. This result can be explained by the existence of an intrinsic peroxidase activity which interfere with the exogenously added enzyme during surface radioiodination.
在有或没有乳过氧化物酶的各种条件下，用葡萄糖氧化酶产生过氧化氢，对刚地弓形虫速殖子进行放射性碘化。同时将红细胞碘化作为对照。在我们的实验条件下，在没有乳过氧化物酶的情况下速殖子标记更强。这一结果可以通过存在一种内在过氧化物酶活性来解释，这种活性在表面放射性碘化过程中会干扰外源添加的酶。
Zotero 插件