Mezl V A, Wall J
Biosci Rep. 1986 May;6(5):429-34. doi: 10.1007/BF01116133.
Amino acid analyses show that while the free amino acids found in the rabbit reticulocyte translation system do not increase during nuclease treatment or during prolonged storage, the endogenous levels of many amino acids are so high that the choice of a radioactive precursor for a translation should be based not only on the abundance of the amino acid in the translation product but also on its concentrations in the lysate preparation. It is shown that the variation of amino acid concentrations reported in this study to calculate the amount of radioactive amino acid necessary for satisfactory incorporation.
氨基酸分析表明，虽然在兔网织红细胞翻译系统中发现的游离氨基酸在核酸酶处理期间或长期储存期间不会增加，但许多氨基酸的内源性水平非常高，以至于选择翻译的放射性前体不仅应基于其在翻译产物中的丰度，还应基于其在裂解物制备中的浓度。结果表明，本研究中报道的氨基酸浓度变化用于计算满意掺入所需的放射性氨基酸量。
