Arnstein H R, Barwick C W, Lange J D, Thomas H D
FEBS Lett. 1986 Jan 1;194(1):146-50. doi: 10.1016/0014-5793(86)80066-7.
The enzyme asparaginase, which hydrolyses asparagine to aspartic acid, inhibited cell-free protein synthesis by reticulocyte lysates. The inhibition was rapid and complete when sufficient enzyme was added but could be prevented or reversed by the addition of asparagine. The initial effect of asparaginase appears to be a block in polypeptide chain elongation due to asparagine deprivation, but there are some indications that prolonged incubation under these conditions may give rise to a secondary decrease in initiation of protein synthesis.
天冬酰胺酶可将天冬酰胺水解成天冬氨酸，它能抑制网织红细胞裂解物的无细胞蛋白质合成。加入足够的酶时，抑制作用迅速且完全，但加入天冬酰胺可防止或逆转这种抑制。天冬酰胺酶的初始作用似乎是由于天冬酰胺缺乏而阻断多肽链的延伸，但有一些迹象表明，在这些条件下长时间孵育可能会导致蛋白质合成起始的继发性减少。
