Biology Department, Federal University of Ceara, Fortaleza, CE, 60440-900, Brazil.
Biochemistry and Molecular Biology Department, Federal University of Ceara, Fortaleza, CE, 60440-900, Brazil.
Inflammopharmacology. 2023 Oct;31(5):2631-2640. doi: 10.1007/s10787-023-01283-0. Epub 2023 Jul 7.
Licania rigida Benth., a Brazilian endemic plant, has been traditionally used for treating inflammation and stomach pain. This work investigates the anti-inflammatory and gastroprotective activities of the ethanolic extract from L. rigida seeds (EELr) by in vitro and in vivo methods. The phytochemical profile was determined and the in vitro antioxidant activity was investigated by radical scavenging and thiobarbituric acid reactive substances methods. The ovalbumin denaturation method was used with sodium diclofenac as standard for the in vitro anti-inflammatory activity assessment. Acetylsalicylic acid was used to induce gastric ulcers in male mice and then to evaluate the preventive and therapeutic gastroprotective effect of EELr, using omeprazole as the reference drug. The extract exhibited relevant amount of phenolic compounds and flavonoids, in particular, demonstrating in vitro antioxidant capacity. EELr was able to inhibit almost 60% of ovalbumin denaturation at a concentration considered low. It also prevented the decrease of biochemical markers for oxidative stress such as superoxide dismutase (SOD) and reduced glutathione (GSH) in the stomach and SOD and catalase (CAT) in the liver. EELr also significantly decreased the number of lesions as well as reduced the ulcerated area when used as therapy. The observed effect may be due to its phenolic compounds, such as chlorogenic acid, caffeic acid and tannins, as previously reported. EELr is a potential source of compounds with anti-inflammatory activity, protects the liver from oxidative damage and improves healing of aspirin-induced ulcers. This work contributes to the knowledge of L. rigida species.
硬胶树，一种巴西特有植物，传统上被用于治疗炎症和胃痛。本工作通过体外和体内方法研究了硬胶树种子乙醇提取物（EELr）的抗炎和胃保护活性。测定了植物化学特征，并通过自由基清除和硫代巴比妥酸反应物质方法研究了体外抗氧化活性。采用卵白蛋白变性法，以双氯芬酸钠为标准，评估体外抗炎活性。采用乙酰水杨酸诱导雄性小鼠胃溃疡，然后用奥美拉唑作为参考药物，评价 EELr 的预防和治疗性胃保护作用。提取物含有大量的酚类化合物和类黄酮，特别是具有体外抗氧化能力。EELr 在低浓度下就能抑制近 60%的卵白蛋白变性。它还能防止胃中氧化应激生化标志物超氧化物歧化酶（SOD）和还原型谷胱甘肽（GSH）以及肝中 SOD 和过氧化氢酶（CAT）的减少。EELr 作为治疗药物时，还能显著减少病变数量和溃疡面积。观察到的作用可能是由于其酚类化合物，如绿原酸、咖啡酸和单宁酸，如前所述。EELr 是具有抗炎活性的化合物的潜在来源，可保护肝脏免受氧化损伤，并有助于阿司匹林诱导的溃疡愈合。本工作为硬胶树属植物的研究做出了贡献。
