Radioimmunoassay of LH-RH. Standard curves and specificity.

As important discrepancies in blood concentrations of LH-RH remain, the basic problem of the specificity of the radioimmunoassay was reinvestigated using an antiserum raised against a new conjugate (33). Cross-reactions with related and unrelated peptides were studied in both charcoal-dextran and second antibody methods of separation. The possible relation between the nature of the conjugate and the specificity is discussed.