Kinugasa T, Tanizawa O, Yamaji K, Kurachi K
Nihon Naibunpi Gakkai Zasshi. 1976 Jan 20;52(1):62-71. doi: 10.1507/endocrine1927.52.1_62.
The radioimmunoassay for LH-RH would aid greatly in the assessment of hypothalamic function. The anti-LH-RH was prepared by immunizing rabbits with LH-RH conjugate of BSA. 125I-LH-RH was prepared by the lactoperoxidase method and Sephadex G-10 column chromatography. The double antibody RIA technique was employed. On the assay of biological materials, LH-RH was extracted by methanol because LH-RH was rapidly destroyed in the serum, and this breakdown could not be prevented by benzamidine or 2,3-dimercaptopropranol. Sensitivity of this RIA system ranged from 10 tp 104 pg/ml, and the coefficient of variations of intra- and interassay were 12.9% and 9.% respectively. The serum LH-RH levels of men in a normal gonad state were below 10 pg/ml, and those of women in a normal gonad state in the early follicular or luteal phase were below 50 pg/ml. Whereas those of postmenopausal or castrated women were increased, and were highest in women with several gonadal disturbed states. The disappearance curve of LH-RH in women was characterized by two exponentials, t(1/2) of the initial component was 4.9 min, and that of the second component 24.6 min. Urinary excretion of exogenously administered LH-RH was also studied.
促黄体生成素释放激素（LH-RH）的放射免疫测定法对评估下丘脑功能有很大帮助。抗LH-RH是通过用牛血清白蛋白（BSA）与LH-RH的结合物免疫兔子制备的。125I-LH-RH采用乳过氧化物酶法和葡聚糖凝胶G-10柱色谱法制备。采用双抗体放射免疫测定技术。在生物材料检测中，由于LH-RH在血清中迅速被破坏，且这种破坏不能被苯甲脒或2,3-二巯基丙醇阻止，所以用甲醇提取LH-RH。该放射免疫测定系统的灵敏度范围为10至104 pg/ml，批内和批间变异系数分别为12.9%和9%。性腺功能正常的男性血清LH-RH水平低于10 pg/ml，卵泡期或黄体期性腺功能正常的女性血清LH-RH水平低于50 pg/ml。而绝经后或去势女性的血清LH-RH水平升高，在几种性腺功能紊乱状态的女性中最高。女性体内LH-RH的消失曲线具有两个指数特征，初始成分的t(1/2)为4.9分钟，第二个成分的t(1/2)为24.6分钟。还对外源性给予的LH-RH的尿排泄情况进行了研究。
