Oliveira Dhaniella Cristhina de Brito, Costa Fernando Henrique Marques, da Silva José Alberto Fracassi
Department of Analytical Chemistry, Institute of Chemistry, State University of Campinas (UNICAMP), P.O. Box 6154, Campinas 13083-970, SP, Brazil.
National Institute of Science and Technology of Bioanalytics, INCTBio, Campinas, SP, Brazil.
Micromachines (Basel). 2023 Mar 31;14(4):791. doi: 10.3390/mi14040791.
Devices that integrate field effect transistors into microfluidic channels are becoming increasingly promising in the medical, environmental, and food realms, among other applications. The uniqueness of this type of sensor lies in its ability to reduce the background signals existing in the measurements, which interfere in obtaining good limits of detection for the target analyte. This and other advantages intensify the development of selective new sensors and biosensors with coupling configuration. This review work focused on the main advances in the fabrication and application of field effect transistors integrated into microfluidic devices as a way of identifying the potentialities that exist in these systems when used in chemical and biochemical analyses. The emergence of research on integrated sensors is not a recent study, although more recently the progress of these devices is more accentuated. Among the studies that used integrated sensors with electrical and microfluidic parts, those that investigated protein binding interactions seem to be the ones that expanded the most due, among other things, to the possibility of obtaining several physicochemical parameters involved in protein-protein interactions. Studies in this area have a great possibility of advancing innovations in sensors with electrical and microfluidic interfaces in new designs and applications.
将场效应晶体管集成到微流控通道中的设备在医学、环境、食品等领域以及其他应用中越来越有前景。这类传感器的独特之处在于它能够减少测量中存在的背景信号，这些背景信号会干扰获得目标分析物的良好检测限。这一优势以及其他优点推动了具有耦合配置的新型选择性传感器和生物传感器的发展。本综述工作聚焦于集成到场效应晶体管微流控设备中的制造和应用方面的主要进展，以此来确定这些系统在用于化学和生化分析时所具有的潜力。集成传感器的研究并非近期才出现，不过最近这些设备的进展更为显著。在使用带有电学和微流控部件的集成传感器的研究中，那些研究蛋白质结合相互作用的研究似乎扩展得最为显著，这尤其得益于获取蛋白质 - 蛋白质相互作用中涉及的多个物理化学参数的可能性。该领域的研究极有可能在新设计和应用中推动具有电学和微流控界面的传感器的创新。
