用于体外膜肺氧合动物模型的新型尺寸可变专用啮齿动物氧合器——“大鼠氧合器”的介绍及初步体外实验结果

Novel Size-Variable Dedicated Rodent Oxygenator for ECLS Animal Models-Introduction of the "RatOx" Oxygenator and Preliminary In Vitro Results.

作者信息

Strudthoff Lasse J, Focke Jannis, Hesselmann Felix, Kaesler Andreas, Martins Costa Ana, Schlanstein Peter C, Schmitz-Rode Thomas, Steinseifer Ulrich, Steuer Niklas B, Wiegmann Bettina, Arens Jutta, Jansen Sebastian V

机构信息

Institute of Applied Medical Engineering, Department of Cardiovascular Engineering, Medical Faculty, RWTH Aachen University, 52074 Aachen, Germany.

Department of Biomechanical Engineering, Faculty of Engineering Technologies, University of Twente, 7522 LW Enschede, The Netherlands.

出版信息

Micromachines (Basel). 2023 Mar 31;14(4):800. doi: 10.3390/mi14040800.

DOI:10.3390/mi14040800

PMID:37421033

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10144398/

Abstract

The overall survival rate of extracorporeal life support (ECLS) remains at 60%. Research and development has been slow, in part due to the lack of sophisticated experimental models. This publication introduces a dedicated rodent oxygenator ("RatOx") and presents preliminary in vitro classification tests. The RatOx has an adaptable fiber module size for various rodent models. Gas transfer performances over the fiber module for different blood flows and fiber module sizes were tested according to DIN EN ISO 7199. At the maximum possible amount of effective fiber surface area and a blood flow of 100 mL/min, the oxygenator performance was tested to a maximum of 6.27 mL O/min and 8.2 mL CO/min, respectively. The priming volume for the largest fiber module is 5.4 mL, while the smallest possible configuration with a single fiber mat layer has a priming volume of 1.1 mL. The novel RatOx ECLS system has been evaluated in vitro and has demonstrated a high degree of compliance with all pre-defined functional criteria for rodent-sized animal models. We intend for the RatOx to become a standard testing platform for scientific studies on ECLS therapy and technology.

摘要

体外生命支持（ECLS）的总体生存率仍为60%。研发进展缓慢，部分原因是缺乏精密的实验模型。本出版物介绍了一种专用的啮齿动物氧合器（“RatOx”），并展示了初步的体外分类测试。RatOx具有适用于各种啮齿动物模型的可适配纤维模块尺寸。根据DIN EN ISO 7199测试了不同血流和纤维模块尺寸下纤维模块的气体传输性能。在有效纤维表面积达到最大可能量且血流为100 mL/分钟时，分别对氧合器性能进行了测试，最高可达6.27 mL O/分钟和8.2 mL CO/分钟。最大纤维模块的预充量为5.4 mL，而具有单纤维垫层的最小可能配置的预充量为1.1 mL。新型RatOx ECLS系统已在体外进行了评估，并已证明高度符合啮齿动物大小动物模型的所有预定义功能标准。我们希望RatOx成为ECLS治疗和技术科学研究的标准测试平台。