Deininger Matthias Manfred, Benner Carl-Friedrich, Strudthoff Lasse Johannes, Leonhardt Steffen, Bruells Christian Simon, Marx Gernot, Bleilevens Christian, Breuer Thomas
Department of Intensive and Intermediate Care, Medical Faculty, RWTH Aachen University, 52074 Aachen, Germany.
Department of Anesthesiology, Medical Faculty, RWTH Aachen University, 52074 Aachen, Germany.
Animals (Basel). 2023 Nov 15;13(22):3532. doi: 10.3390/ani13223532.
The development of biomedical soft- or hardware frequently includes testing in animals. However, large efforts have been made to reduce the number of animal experiments, according to the 3Rs principle. Simultaneously, a significant number of surplus animals are euthanized without scientific necessity. The primary aim of this study was to establish a post-mortem rat perfusion model using extracorporeal membrane oxygenation (ECMO) in surplus rat cadavers and generate first post vivo results concerning the oxygenation performance of a recently developed ECMO membrane oxygenator. Four rats were euthanized and connected post-mortem to a venous-arterial ECMO circulation for up to eight hours. Angiographic perfusion proofs, blood gas analyses and blood oxygenation calculations were performed. The mean preparation time for the ECMO system was 791 ± 29 s and sufficient organ perfusion could be maintained for 463 ± 26 min, proofed via angiographic imaging and a mean femoral arterial pressure of 43 ± 17 mmHg. A stable partial oxygen pressure, a 73% rise in arterial oxygen concentration and an exponentially increasing oxygen extraction ratio up to 4.75 times were shown. Considering the 3Rs, the established post-mortal ECMO perfusion rat model using surplus animals represents a promising alternative to models using live animals. Given the preserved organ perfusion, its use could be conceivable for various biomedical device testing.
生物医学软硬件的开发常常包括动物实验。然而，根据3R原则，人们已做出巨大努力来减少动物实验的数量。与此同时，大量多余的动物被无科学必要地处死。本研究的主要目的是在多余的大鼠尸体上建立使用体外膜肺氧合（ECMO）的大鼠死后灌注模型，并得出关于一种最新开发的ECMO膜式氧合器氧合性能的首个体内后结果。对四只大鼠实施安乐死后，将其与静脉-动脉ECMO循环连接长达八小时。进行了血管造影灌注验证、血气分析和血液氧合计算。ECMO系统的平均准备时间为791±29秒，通过血管造影成像和平均股动脉压43±17毫米汞柱证明，可维持463±26分钟的充足器官灌注。结果显示氧分压稳定，动脉氧浓度上升73%，氧摄取率呈指数增长，最高达4.75倍。考虑到3R原则，利用多余动物建立的死后ECMO灌注大鼠模型是使用活体动物模型的一个有前景的替代方案。鉴于器官灌注得以保留，其可设想用于各种生物医学设备测试。
