Department of Periodontology, Hadassah Medical Center, Faculty of Dental Medicine, Hebrew University of Jerusalem, Jerusalem, Israel.
Robert K. Schenk Laboratory of Oral Histology, Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland.
Clin Oral Investig. 2023 Sep;27(9):5041-5048. doi: 10.1007/s00784-023-05123-x. Epub 2023 Jul 8.
To histologically evaluate the effects of a novel human recombinant amelogenin (rAmelX) on periodontal wound healing / regeneration in recession-type defects.
A total of 17 gingival recession-type defects were surgically created in the maxilla of three minipigs. The defects were randomly treated with a coronally advanced flap (CAF) and either rAmelX (test), or a CAF and placebo (control). At three months following reconstructive surgery, the animals were euthanized, and the healing outcomes histologically evaluated.
The test group yielded statistically significantly (p = 0.047) greater formation of cementum with inserting collagen fibers compared with the control group (i.e., 4.38 mm ± 0.36 mm vs. 3.48 mm ± 1.13 mm). Bone formation measured 2.15 mm ± 0.8 mm in the test group and 2.24 mm ± 1.23 mm in the control group, respectively, without a statistically significant difference (p = 0.94).
The present data have provided for the first-time evidence for the potential of rAmelX to promote regeneration of periodontal ligament and root cementum in recession-type defects, thus warranting further preclinical and clinical testing.
The present results set the basis for the potential clinical application of rAmelX in reconstructive periodontal surgery.
组织学评估新型人重组釉原蛋白（rAmelX）对牙周退缩型缺损中牙周愈合/再生的影响。
在三只小型猪的上颌中总共创建了 17 个牙周退缩型缺损。这些缺损被随机用冠向推进瓣（CAF）处理，并用 rAmelX（实验组）或 CAF 和安慰剂（对照组）处理。在重建手术后三个月，处死动物，并对愈合结果进行组织学评估。
实验组与对照组相比，牙骨质中插入的胶原纤维形成有统计学显著差异（p=0.047）（即 4.38±0.36mm 比 3.48±1.13mm）。实验组的骨形成量为 2.15±0.8mm，对照组为 2.24±1.23mm，无统计学显著差异（p=0.94）。
本研究首次提供了 rAmelX 促进牙周退缩型缺损中牙周韧带和牙根牙骨质再生潜力的证据，因此需要进一步进行临床前和临床测试。
本研究结果为 rAmelX 在牙周重建手术中的潜在临床应用奠定了基础。
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