Fritz H, Kruck J, Rüsse I, Liebich H G
Hoppe Seylers Z Physiol Chem. 1979 Mar;360(3):437-44. doi: 10.1515/bchm2.1979.360.1.437.
Using the indirect immunofluorescence technique, the basic kallikrein-trypsin inhibitor of bovine organs, Trasylol, could be localized in tissue mast cells of bovine lung, liver, pancreas and parotid gland. Identification of cells exhibiting specific fluorescence as tissue mast cells was achieved by combined light and electron microscopic diagnosis of bovine liver tissue sections. The presence of Trasylol in mast cells explains the widespread distribution of this inhibitor in functionally totally different organs or tissues of the bovine organism, as determined earlier by biochemical means. Identification of Trasylol as a mast cell constituent will facilitate the search for the biological function of this inhibitory protein in connection with a unique and highly specialized cell population.
采用间接免疫荧光技术，可将牛器官的碱性激肽释放酶 - 胰蛋白酶抑制剂（抑肽酶）定位在牛肺、肝、胰腺和腮腺的组织肥大细胞中。通过对牛肝组织切片进行光学显微镜和电子显微镜联合诊断，将呈现特异性荧光的细胞鉴定为组织肥大细胞。肥大细胞中抑肽酶的存在解释了该抑制剂在牛机体功能完全不同的器官或组织中的广泛分布，这一点在之前已通过生化方法得以确定。将抑肽酶鉴定为肥大细胞的一种成分，将有助于探寻这种抑制蛋白与独特且高度特化的细胞群体相关的生物学功能。
