Shikimi T, Kobayashi T
J Pharmacobiodyn. 1980 Aug;3(8):400-6. doi: 10.1248/bpb1978.3.400.
Antibody to aprotinin was easily produced in rabbits by injecting an emulsion of Trasylol and adjuvant, and its specificity was confirmed by double diffusion, immunoelectrophoresis in gel, passive hemagglutination and affinity chromatography of Trasylol. The location of aprotinin in bovine lung and pancreas was determined by an indirect fluorescent antibody technique. The fluorescence observed in the connective tissues was found to be due to the mononuclear cells in these tissues.
通过注射抑肽酶乳剂和佐剂，很容易在兔体内产生抗抑肽酶抗体，其特异性通过双向扩散、凝胶免疫电泳、被动血凝以及抑肽酶的亲和层析得以证实。采用间接荧光抗体技术确定了抑肽酶在牛肺和胰腺中的定位。发现结缔组织中观察到的荧光是由于这些组织中的单核细胞所致。
