Escuela de Graduados, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile.
Instituto de Ciencias Clínicas Veterinarias, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile.
J Equine Vet Sci. 2023 Sep;128:104881. doi: 10.1016/j.jevs.2023.104881. Epub 2023 Jul 6.
Microminerals are necessary for all bodily functions. In animal species, selenium (Se), copper (Cu) and zinc (Zn) all form part of antioxidant enzymes. Micromineral deficiencies, particularly Se, are well recognized in large animal species in Chile. Glutathion peroxidase (GPx) is a widely used biomarker for Se nutritional status and to diagnose Se deficiency in horses. Superoxide dismutase (SOD) is a Cu and Zn-dependant antioxidant enzyme, although it is not commonly used as a proxy for the nutritional status of these minerals. Ceruloplasmin (CP) is used as a biomarker of Cu nutritional status. This study´s objective was to explore correlation between said minerals and biomarkers in adult horses from southern Chile. Se, Cu, Zn, GPx, SOD, and CP were measured in whole blood of a group of 32 adult horses (5-15 years old). Further, a second group of 14 adult horses (5-15 years old) underwent gluteal muscle biopsies to determine Cu, Zn, GPx, and SOD. Correlations were determined by means of Pearson´s r coefficient. Significant correlations were found for blood GPx and Se (r = 0.79), blood GPx and SOD (r = -0.6), muscular GPx and SOD (r = 0.78), and Cu and CP (r = 0.48). These results confirm previously described strong association between blood GPx and Se in horses, validating the former´s use as a diagnostic proxy of Se deficiency in Chilean horses, and suggest significant interactions between GPx and SOD in blood and muscle tissue.
微量元素对所有身体功能都是必需的。在动物物种中，硒（Se）、铜（Cu）和锌（Zn）都是抗氧化酶的组成部分。在智利的大型动物物种中，已经充分认识到微量元素缺乏，特别是硒缺乏。谷胱甘肽过氧化物酶（GPx）是一种广泛用于评估硒营养状况和诊断马硒缺乏的生物标志物。超氧化物歧化酶（SOD）是一种依赖 Cu 和 Zn 的抗氧化酶，尽管它通常不作为这些矿物质营养状况的替代指标。铜蓝蛋白（CP）被用作 Cu 营养状况的生物标志物。本研究的目的是探讨智利南部成年马中这些矿物质和生物标志物之间的相关性。对一组 32 匹成年马（5-15 岁）的全血进行了 Se、Cu、Zn、GPx、SOD 和 CP 测量。此外，第二组 14 匹成年马（5-15 岁）进行了臀肌活检，以确定 Cu、Zn、GPx 和 SOD。通过 Pearson r 系数确定相关性。血液 GPx 与 Se（r=0.79）、血液 GPx 与 SOD（r=-0.6）、肌肉 GPx 与 SOD（r=0.78）和 Cu 与 CP（r=0.48）之间存在显著相关性。这些结果证实了以前在马中描述的血液 GPx 与 Se 之间的强烈关联，验证了前者在智利马中作为 Se 缺乏的诊断替代指标的使用，并表明血液和肌肉组织中 GPx 和 SOD 之间存在显著相互作用。
