Kirschvink N, de Moffarts B, Farnir F, Pincemail J, Lekeux P
Department for Functional Sciences, Department for Animal Productions, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium.
Equine Vet J Suppl. 2006 Aug(36):239-44. doi: 10.1111/j.2042-3306.2006.tb05546.x.
There is increasing evidence that the equine athlete is exposed to exercise-induced changes of its oxidant/antioxidant balance and antioxidant supplementation is frequently recommended. However, it is unknown whether there is a specific need for antioxidants according to performance, breed, gender or age.
To assess whether breed-, gender- and age-related differences of blood oxidant/antioxidant markers occur in competition horses.
Healthy horses (n = 493) underwent oxidant/ antioxidant blood marker determination. Vitamin E, lipophilic antioxidant capacity (ACL), ascorbic acid (AA), glutathione (GSH, GSSG), gluthione peroxidase (GPx), superoxide dismutase (SOD), selenium (Se), copper (Cu), zinc (Zn), lipid peroxides (Pool), oxidised proteins (Protox) were determined, as well as magnesium (Mg), creatine phosphokinase (CPK), lactate dehydrogenase (LDH), packed cell volume (PCV) and haemoglobin (Hb). A mixed linear model assessed the effect of breed, gender and age category. P<0.05 was considered significant.
Thoroughbreds showed the highest values of vitamin E, ACL, GPx, PCV and Hb, whilst standardbreds had the highest values of AA and LDH. Jumping horses had the highest Protox values. Females had significantly higher SOD values, whereas most of the other markers were higher in stallions and geldings. Horses age 2-6 years had higher AA, SOD and LDH values than horses age >6 years. Correlation analyses were positive and significant between vitamin E and GPx, VitE and ACL, Se and GPx, Cu and Pool and negative between Pool and vitamin E, Pool and ACL, Protox and GPx, Protox and vitamin E.
Blood oxidant/ antioxidant status of horses is influenced by breed, gender and age. The correlation analyses suggest synergistic relations between GPx, vitamin E and Se and an antagonistic relation between Protox-GPx, Protox-vitamin E, and Pool-vitamin E.
The results of this investigation provide definition of the specific need for antioxidants and vitamins in competition horses.
越来越多的证据表明，马运动员会受到运动诱导的氧化/抗氧化平衡变化的影响，因此经常建议补充抗氧化剂。然而，根据运动表现、品种、性别或年龄对抗氧化剂是否有特定需求尚不清楚。
评估参赛马匹血液中氧化/抗氧化标志物是否存在与品种、性别和年龄相关的差异。
对493匹健康马匹进行氧化/抗氧化血液标志物测定。测定了维生素E、亲脂性抗氧化能力（ACL）、抗坏血酸（AA）、谷胱甘肽（GSH、GSSG）、谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）、硒（Se）、铜（Cu）、锌（Zn）、脂质过氧化物（Pool）、氧化蛋白（Protox），以及镁（Mg）、肌酸磷酸激酶（CPK）、乳酸脱氢酶（LDH）、红细胞压积（PCV）和血红蛋白（Hb）。采用混合线性模型评估品种、性别和年龄类别的影响。P<0.05被认为具有显著性。
纯种马的维生素E、ACL、GPx、PCV和Hb值最高，而标准赛马的AA和LDH值最高。跳跃马的Protox值最高。雌性的SOD值显著更高，而大多数其他标志物在种马和去势雄马中更高。2至6岁的马比6岁以上的马具有更高的AA、SOD和LDH值。维生素E与GPx、维生素E与ACL、硒与GPx、铜与Pool之间的相关性分析呈正且显著，而Pool与维生素E、Pool与ACL、Protox与GPx、Protox与维生素E之间呈负相关。
马的血液氧化/抗氧化状态受品种、性别和年龄影响。相关性分析表明，GPx、维生素E和硒之间存在协同关系，而Protox-GPx、Protox-维生素E和Pool-维生素E之间存在拮抗关系。
本研究结果明确了参赛马匹对抗氧化剂和维生素的特定需求。
