Laboratory of Virology, Microbiology Department, Hospital Universitario, 12 de Octubre, Avda de Córdoba s/n, 28041, Madrid, Spain.
Biomedical Research Institute imas12, Hospital Universitario 12 de Octubre, Madrid, Spain.
Sci Rep. 2023 Jul 8;13(1):11094. doi: 10.1038/s41598-023-37866-8.
The utility of reverse transcription-polymerase chain reaction (RT-PCR) in analysis SARS-COV-2 variants was evaluated. RT-PCR tests were used to analyse the majority of new SARS-CoV-2 cases (n = 9315) in a tertiary hospital (Madrid, Spain) throughout 2021. Subsequently, whole genome sequencing (WGS) was conducted on 10.8% of these samples (n = 1002). Notably, the Delta and Omicron variants emerged rapidly. There were no discrepancies between RT-PCR and WGS results. Continuous surveillance of SARS-CoV-2 variants is essential, and RT-PCR is a highly useful method, specially during periods of high COVID-19 incidence. This feasible technique can be implemented in all SARS-CoV-2 laboratories. However, WGS remains the gold standard method for comprehensive detection of all existing SARS-CoV-2 variants.
评估了逆转录聚合酶链反应 (RT-PCR) 在分析 SARS-CoV-2 变体中的效用。RT-PCR 测试用于分析 2021 年在一家三级医院（西班牙马德里）的大多数新 SARS-CoV-2 病例（n=9315）。随后，对这些样本中的 10.8%（n=1002）进行了全基因组测序 (WGS)。值得注意的是，Delta 和 Omicron 变体迅速出现。RT-PCR 和 WGS 结果之间没有差异。对 SARS-CoV-2 变体的持续监测至关重要，RT-PCR 是一种非常有用的方法，特别是在 COVID-19 发病率高的时期。这种可行的技术可以在所有 SARS-CoV-2 实验室中实施。然而，WGS 仍然是全面检测所有现有 SARS-CoV-2 变体的金标准方法。
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