Department of Neurosurgery, The First Hospital of China Medical University, Shenyang, China.
Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang, China.
Front Endocrinol (Lausanne). 2023 Jun 22;14:1151714. doi: 10.3389/fendo.2023.1151714. eCollection 2023.
To investigate the levels of tumor-infiltrating CD8 lymphocytes (CD8 TILs) and the expression of programmed cell death receptor ligand 1 (PD-L1) in the tumor microenvironment (TME) of pediatric and adolescent pituitary adenomas (PAPAs) and analyze the correlation between their levels and the clinical characteristics.
A series of 43 PAPAs cases were enrolled over a period of 5 years. To compare the TME of PAPAs and adult PAs, 43 PAPAs cases were matched with 60 adult PAs cases (30 cases were between 20 and 40 years old, and 30 cases were older than 40 years) for main clinical characteristics. The expression of immune markers in PAPAs was detected by immunohistochemistry, and their correlation with the clinical outcomes was analyzed using statistical methods.
In the PAPAs group, CD8 TILs level was significantly lower (3.4 (5.7) vs. 6.1 (8.5), p = 0.001), and PD-L1 expression (0.040 (0.022) vs. 0.024 (0.024), p < 0.0001) was significantly higher as compared with the older group. The level of CD8 TILs was negatively correlated with the expression of PD-L1 (r = -0.312, p = 0.042). Moreover, CD8 TILs and PD-L1 levels were associated with Hardy (CD8, p = 0.014; PD-L1, p = 0.018) and Knosp (CD8, p = 0.02; PD-L1, p = 0.017) classification. CD8 TILs level was associated with high-risk adenomas (p = 0.015), and it was associated with the recurrence of PAPAs (HR = 0.047, 95% CI 0.003-0.632, p = 0.021).
Compared with the TME in adult PAs, the TME in PAPAs was found to express a significantly altered level of CD8 TILs and PD-L1. In PAPAs, CD8 TILs and PD-L1 levels were associated with clinical characteristics.
研究儿童和青少年垂体腺瘤（PAPA）肿瘤微环境（TME）中肿瘤浸润性 CD8 淋巴细胞（CD8 TILs）水平和程序性死亡受体配体 1（PD-L1）的表达，并分析其水平与临床特征的相关性。
本研究纳入了 5 年内的 43 例 PAPA 病例。为了比较 PAPA 和成人 PAs 的 TME，将 43 例 PAPA 病例与 60 例成人 PAs 病例（20-40 岁 30 例，>40 岁 30 例）进行主要临床特征匹配。采用免疫组织化学法检测 PAPA 中免疫标志物的表达，并采用统计学方法分析其与临床结局的相关性。
在 PAPA 组中，CD8 TILs 水平明显较低（3.4（5.7）vs. 6.1（8.5），p = 0.001），PD-L1 表达（0.040（0.022）vs. 0.024（0.024），p < 0.0001）明显较高。CD8 TILs 水平与 PD-L1 表达呈负相关（r = -0.312，p = 0.042）。此外，CD8 TILs 和 PD-L1 水平与 Hardy（CD8，p = 0.014；PD-L1，p = 0.018）和 Knosp（CD8，p = 0.02；PD-L1，p = 0.017）分级相关。CD8 TILs 水平与高危腺瘤有关（p = 0.015），与 PAPA 复发有关（HR = 0.047，95%CI 0.003-0.632，p = 0.021）。
与成人 PAs 的 TME 相比，PAPA 的 TME 中 CD8 TILs 和 PD-L1 的表达水平明显改变。在 PAPA 中，CD8 TILs 和 PD-L1 水平与临床特征相关。
