Namima Tomoyuki, Kempkes Erin, Smith Bob, Pasupathy Anitha
Department of Biological Structure and Washington National Primate Research Center, University of Washington, Seattle, WA 98195, USA.
Graduate School of Frontier Biosciences, Osaka University, and Center for Information and Neural Networks, National Institute of Information and Communications Technology, Suita, Osaka, 565-0871, Japan.
bioRxiv. 2023 Jun 29:2023.06.26.546631. doi: 10.1101/2023.06.26.546631.
Neuropixels probes have revolutionized neurophysiological studies in the rodent, but inserting these probes through the much thicker primate dura remains a challenge. Here we describe two methods we have developed for the insertion of two types of Neuropixels probes acutely into the awake monkey cortex. For the fine rodent probe, which is unable to pierce native primate dura, we developed a dural-eyelet method to insert the probe repeatedly without breakage. For the thicker NHP probe, we developed an artificial dura system to insert the probe. We have now conducted successful experiments in 3 animals across 7 recording chambers with the procedures described here and have achieved stable recordings over several months in each case. Here we describe our hardware, surgical preparation, methods for insertion and methods for removal of broken probe parts. We hope that our methods are of value to primate physiologists everywhere.
神经像素探针彻底改变了啮齿动物的神经生理学研究，但将这些探针插入厚得多的灵长类动物硬脑膜仍然是一项挑战。在这里，我们描述了我们开发的两种方法，用于将两种类型的神经像素探针急性插入清醒猴子的皮层。对于无法穿透天然灵长类动物硬脑膜的精细啮齿动物探针，我们开发了一种硬脑膜小孔方法，以便在不损坏的情况下重复插入探针。对于更厚的非人灵长类动物探针，我们开发了一种人工硬脑膜系统来插入探针。我们现在已经使用这里描述的程序在3只动物的7个记录室中成功进行了实验，并且在每种情况下都实现了数月的稳定记录。在这里，我们描述了我们的硬件、手术准备、插入方法和取出断裂探针部件的方法。我们希望我们的方法对各地的灵长类动物生理学家有价值。
