Campbell P B, Tolson T A
Cell Immunol. 1986 Jan;97(1):67-79. doi: 10.1016/0008-8749(86)90376-x.
The leukotactic responsiveness of human peripheral blood monocytes is regulated by the cell-directed inhibitor of monocyte leukotaxis, CDI-MLx. Normal peripheral blood mononuclear cells elaborated CDI-MLx in vitro in response to soluble and cell-associated antigens; concanavalin A and pokeweed mitogen induced production of the inhibitor while phytohemagglutinin and staphylococcal protein A were without effect. By erythrocyte rosetting and immunoadherence, the CDI-producing cell had the phenotype, E-rosette+, OKMI+, and Leu 7+, and thus appeared related to natural killer cells. PBMC and plasma CDI-MLx had similar molecular weights (230,000) and showed similar heterogeneity on chromatofocusing with peaks of activity at pH 5.8 and 5.25. CDI-MLx was immunologically distinct from immunoglobulins, did not inactivate preformed leukotaxins, and was distinct from other lymphokines known to modulate monocyte locomotion.
人外周血单核细胞的白细胞趋化反应性受单核细胞白细胞趋化作用的细胞定向抑制剂CDI-MLx调节。正常外周血单个核细胞在体外对可溶性和细胞相关抗原产生反应时会产生CDI-MLx；伴刀豆球蛋白A和商陆有丝分裂原可诱导该抑制剂的产生，而植物血凝素和葡萄球菌蛋白A则无此作用。通过红细胞玫瑰花结形成试验和免疫黏附试验，产生CDI的细胞具有E玫瑰花结阳性、OKM1阳性和Leu 7阳性的表型，因此似乎与自然杀伤细胞有关。外周血单个核细胞（PBMC）和血浆中的CDI-MLx具有相似的分子量（230,000），并且在色谱聚焦上显示出相似的异质性，活性峰出现在pH 5.8和5.25处。CDI-MLx在免疫学上与免疫球蛋白不同，不会使预先形成的白细胞趋化因子失活，并且与已知调节单核细胞运动的其他淋巴因子不同。
