Campbell P B, Tolson T A
Am Rev Respir Dis. 1986 Nov;134(5):1029-35. doi: 10.1164/arrd.1986.134.5.1029.
Sarcoidosis is an immunopathogenic disorder of uncertain cause. Because the regulation of monocyte mobilization and function may be critical to granuloma formation in this disease, we evaluated production of the cell-directed inhibitor of monocyte leukotaxis (CDI-MLx) by peripheral blood and bronchoalveolar lavage mononuclear cells from these patients. Cells obtained from either source during clinically active disease spontaneously produced this leukotactic regulator in vitro in amounts comparable to those achieved with maximal mitogenic stimulation of normal cells. Plasma leukotactic inhibitory activity and spontaneous inhibitor production were significantly associated. Plasma inhibitory activity and CDI-MLx production were normal in patients whose disease was inactive. Spontaneous production continued for at least 7 days in vitro and could not be attributed to alterations in the absolute numbers of specific mononuclear cell populations. Partitioning of peripheral blood mononuclear cells by a combination of E-rosetting and immunoadherence techniques indicated, however, that CDI-MLx was produced by a subpopulation of natural killer-like cells that formed E-rosettes and bore the OKMI and Leu 7 membrane antigens.
结节病是一种病因不明的免疫病理紊乱疾病。由于单核细胞动员和功能的调节可能对该疾病中肉芽肿的形成至关重要，我们评估了这些患者外周血和支气管肺泡灌洗单核细胞产生单核细胞趋化性细胞定向抑制剂（CDI-MLx）的情况。在临床活动期从任一来源获取的细胞在体外自发产生这种趋化调节因子，其数量与正常细胞最大促有丝分裂刺激所达到的数量相当。血浆趋化抑制活性与自发抑制剂产生显著相关。疾病无活动的患者血浆抑制活性和CDI-MLx产生正常。自发产生在体外持续至少7天，且不能归因于特定单核细胞群体绝对数量的改变。然而，通过E花环形成和免疫黏附技术联合对外周血单核细胞进行分离表明，CDI-MLx由形成E花环且带有OKMI和Leu 7膜抗原的自然杀伤样细胞亚群产生。
