Goldsobel A, Ank B, Spina C, Giorgi J, Stiehm E R
Cell Immunol. 1986 Feb;97(2):335-43. doi: 10.1016/0008-8749(86)90404-1.
Despite some functional impairment of the newborn's T-cell immune system, most infants survive the intrauterine and perinatal period without succumbing to infection or maternal lymphocyte engraftment. The placenta may play a crucial role in protecting the infant from microbial and histocompatibility antigens. Accordingly, we studied phenotypic and functional capacities of placental cells. Placentas were obtained from uncomplicated pregnancies. Matched cord blood and maternal peripheral blood were also obtained in many instances. Fresh minced placental tissue was washed and digested with collagenase and DNase and mononuclear cells were obtained by density gradient centrifugation. The average yield was 10(6) cells/g of tissue with greater than 80% viability. Chromosome analysis of five placental preparations indicated that these cells were of fetal rather than maternal origin. The isolated placental cells consisted of trophoblasts, lymphocytes (74 +/- 3%), monocytes (16 +/- 3%), and granulocytes (8 +/- 2%). E-rosette forming cells (T cells) made up 65 +/- 2% and surface membrane immunoglobulin positive cells made up 8 +/- 1% of the placental mononuclear cells. Fluorescent activated analysis of the mononuclear cells indicated less Leu 4-positive cells (Pan-T) 43 +/- 3%, and less Leu 3-positive (T-helper cells) (25 +/- 2%), than cord and maternal cell preparations. Leu-2, DR, and B1 positive cells were similar to those in cord and maternal blood. Leu 7 and especially Leu 11 positive cells, markers for natural killer cells, were abundant in placental cells, making up 4 +/- 0.7% and 20 +/- 3%, respectively. The Leu 7/Leu 11 ratio of the placental cells was different from either the maternal or cord blood cells. Natural killer activity of placental cells against a K562 natural killer target was low, despite the abundance of cells with NK markers. The K562 activity was low in the placental cells, similar to the low NK activity of maternal and cord cells. Molt 4f killer activity was near normal. Lectin-dependent cytotoxicity using an EL-4 cell target plus PHA was low in placentas, compared to normal, maternal, or cord cell cytotoxicity. Matched samples indicated that LDCC activity was mother greater than cord greater than placenta. Antibody-dependent cytotoxicity (Raji target) of placental cells showed low activity, and again the paired studies indicated that normal controls greater than maternal greater than cord greater than placenta cytotoxicity.(ABSTRACT TRUNCATED AT 400 WORDS)
尽管新生儿的T细胞免疫系统存在一些功能损害，但大多数婴儿在宫内和围产期存活下来，未感染或未发生母体淋巴细胞植入。胎盘可能在保护婴儿免受微生物和组织相容性抗原侵害方面发挥关键作用。因此，我们研究了胎盘细胞的表型和功能能力。胎盘取自正常妊娠。在许多情况下还获取了配对的脐带血和母体外周血。将新鲜切碎的胎盘组织洗涤后用胶原酶和DNA酶消化，通过密度梯度离心获得单核细胞。平均产量为每克组织10⁶个细胞，活力大于80%。对五份胎盘制剂进行的染色体分析表明，这些细胞来源于胎儿而非母体。分离出的胎盘细胞由滋养层细胞、淋巴细胞（74±3%）、单核细胞（16±3%）和粒细胞（8±2%）组成。E花环形成细胞（T细胞）占胎盘单核细胞的65±2%，表面膜免疫球蛋白阳性细胞占8±1%。对单核细胞的荧光激活分析表明，与脐带血和母体细胞制剂相比，Leu 4阳性细胞（泛T细胞）较少（43±3%），Leu 3阳性细胞（辅助性T细胞）较少（25±2%）。Leu - 2、DR和B1阳性细胞与脐带血和母体血液中的相似。Leu 7尤其是Leu 11阳性细胞，作为自然杀伤细胞的标志物，在胎盘细胞中含量丰富，分别占4±0.7%和20±3%。胎盘细胞的Leu 7/Leu 11比值与母体或脐带血细胞不同。尽管具有NK标志物的细胞数量丰富，但胎盘细胞对K562自然杀伤靶标的自然杀伤活性较低。胎盘细胞中的K562活性较低，与母体和脐带血细胞的低NK活性相似。Molt 4f杀伤活性接近正常。与正常、母体或脐带细胞的细胞毒性相比，使用EL - 4细胞靶标加PHA的凝集素依赖性细胞毒性在胎盘中较低。配对样本表明，凝集素依赖性细胞毒性活性为母体大于脐带血大于胎盘。胎盘细胞的抗体依赖性细胞毒性（Raji靶标）显示活性较低，配对研究再次表明，细胞毒性为正常对照大于母体大于脐带血大于胎盘。（摘要截取自400字）
