Department of Biological Sciences, University of Kabianga, Kericho, Kenya.
Pan Afr Med J. 2023 May 5;45:19. doi: 10.11604/pamj.2023.45.19.19585. eCollection 2023.
urinary tract infection (UTI) comes second after respiratory infections in most communities and hospital settings, affecting people of all ages. Frequent use of antibiotics to manage UTI has resulted in development of resistance, calling upon policymakers to fast-track and enforce policies that guide the use of antibiotics in the country. This study intended to determine the current antibiotic resistance to uropathogens among patients attending Kericho County Referral Hospital.
three hundred urine samples from eligible participants were cultured and bacteria colonies identified using biochemical tests. Antibiotic sensitivity was done using Kirby Bauer disk diffusion method on Mueller Hinton Agar. the aetiological agents of UTI were Staphylococcus aureus, Enterococci faecalis, E. coli, Proteus spp and Klebsiella pneumonia. Antibiotic resistance was observed among these uropathogens to commonly used antibiotics namely; ampicillin (84.3%), azithromycin (71.9%) and augmentin (69.8%). However, there were some bacteria that were susceptible to all or some commonly used antibiotics. There was moderate resistance to norfloxacin (43%) except in Staphylococcus aureus which showed 64% resistance. The isolates showed less resistance to cefoxitine (13.2%), gentamycin (11.6%) and ciprofloxacin (10%). While most bacteria showed multiple resistance to 3 drugs, some showed resistance to at most 5 drugs tested in the study. this study found Staphylococcus aureus to be the predominant aetiological agent of UTI. Cefoxitine, gentamycin and ciprofloxacin are good therapeutic choices for confirmed recurrent UTI when culture results are unavailable. There is need to have regular screening of aetiological agents of UTI and their resistance to antimicrobials.
在大多数社区和医院环境中，尿路感染 (UTI) 是继呼吸道感染之后的第二大疾病，影响着各个年龄段的人群。为了治疗 UTI 而频繁使用抗生素导致了耐药性的产生，这促使政策制定者加快并执行指导该国抗生素使用的政策。本研究旨在确定肯尼亚克里乔县转诊医院就诊患者的尿路病原体当前对抗生素的耐药性。
从符合条件的参与者中采集 300 份尿液样本进行培养，并使用生化试验鉴定细菌菌落。使用 Kirby Bauer 磁盘扩散法在 Mueller Hinton 琼脂上进行抗生素敏感性测试。UTI 的病原体是金黄色葡萄球菌、粪肠球菌、大肠杆菌、变形杆菌和肺炎克雷伯菌。观察这些尿路病原体对常用抗生素的抗生素耐药性，包括氨苄西林（84.3%）、阿奇霉素（71.9%）和复方新诺明（69.8%）。然而，有些细菌对所有或某些常用抗生素敏感。除了金黄色葡萄球菌（64%），诺氟沙星（43%）表现出中度耐药性。该分离株对头孢西丁（13.2%）、庆大霉素（11.6%）和环丙沙星（10%）的耐药性较低。虽然大多数细菌对 3 种药物表现出多重耐药性，但有些细菌对研究中测试的最多 5 种药物表现出耐药性。本研究发现金黄色葡萄球菌是 UTI 的主要病原体。当培养结果不可用时，头孢西丁、庆大霉素和环丙沙星是确诊复发性 UTI 的良好治疗选择。有必要定期筛查 UTI 的病原体及其对抗微生物药物的耐药性。
