Randerath K, Reddy M V, Disher R M
Carcinogenesis. 1986 Sep;7(9):1615-7. doi: 10.1093/carcin/7.9.1615.
When liver, kidney, lung and heart DNA preparations of untreated Sprague-Dawley rats of different ages (3 days-10 months) were analyzed for the possible presence of covalent modifications by a 32P-postlabeling assay, characteristic tissue-specific patterns of 32P-labeled spots (termed I-spots) were observed on thin-layer chromatograms. Amounts of these DNA derivatives (termed I-compounds), which were not detected in newborn rat DNA, markedly increased with age. This novel type of DNA modification could be due to environmental (e.g. dietary) factors or to endogenous DNA-reactive metabolites and may conceivably play a role in the initiation of spontaneous cancers or other adverse health effects related to aging.
当采用³²P后标记分析法分析不同年龄（3天至10个月）未处理的斯普拉格-道利大鼠的肝脏、肾脏、肺和心脏DNA制剂中可能存在的共价修饰时，在薄层色谱图上观察到了³²P标记斑点（称为I斑点）的特征性组织特异性模式。这些在新生大鼠DNA中未检测到的DNA衍生物（称为I化合物）的量随着年龄的增长而显著增加。这种新型的DNA修饰可能是由于环境（如饮食）因素或内源性DNA反应性代谢产物引起的，并且可以想象在自发癌症的起始或与衰老相关的其他不良健康影响中发挥作用。
