Pan Lina, Gao Wenfang, Liu Xinyu, Qin Dongyu, Zhang Tiantian, Ren Rui, Zhang Weiyi, Sun Meidi, Gao Cuiqing, Bai Penghua, You Wei, Zhu Gengping, Li Min
Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity, Tianjin Normal University, Tianjin, China.
Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing, China.
Pest Manag Sci. 2023 Nov;79(11):4547-4556. doi: 10.1002/ps.7656. Epub 2023 Aug 7.
How parasitoids distinguish hosts from non-hosts remains an unknown question. Chouioia cunea Yang (Eulophidae) is an important fall webworm parasitoid that attacks many forest and agricultural pests. To study the differences in the chemical clues used by C. cunea to distinguish host and non-host plants, we used gas chromatography-mass spectrometry (GC-MS) to identify volatile compounds of two C. cunea hosts (Hyphantria cunea and Helicoverpa armigera) and two non-hosts (Spodoptera exigua and Spodoptera frugiperda). Additionally, we used behavioral assays to compare the attraction of C. cunea to various compounds.
The two natural host species were more attractive than the two non-host species, in the following order: Hyphantria cunea > Helicoverpa armigera > S. exigua = S. frugiperda. The pupae of the natural hosts contained 1-dodecene, which was not produced by the two natural non-hosts. When the 'attractants' based on the difference between the species-specific blend emitted by pupae and the optimal blend were sprayed onto the natural non-host pupae, they significantly improved the attraction of C. cunea to the non-host pupae.
These results revealed that specific host-produced volatile compounds guide C. cunea to distinguish between natural hosts and non-hosts. Overall, this study provides a foundation for developing a behavior-modifying strategy to re-direct C. cunea attacks to control important non-host pests. © 2023 Society of Chemical Industry.
寄生蜂如何区分宿主和非宿主仍是一个未知问题。白蛾周氏啮小蜂（姬小蜂科）是一种重要的美国白蛾寄生蜂，可攻击多种森林和农业害虫。为研究白蛾周氏啮小蜂用于区分宿主植物和非宿主植物的化学线索差异，我们采用气相色谱 - 质谱联用仪（GC-MS）鉴定了两种白蛾周氏啮小蜂宿主（美国白蛾和棉铃虫）以及两种非宿主（甜菜夜蛾和草地贪夜蛾）的挥发性化合物。此外，我们通过行为测定比较了白蛾周氏啮小蜂对各种化合物的吸引力。
两种天然宿主物种比两种非宿主物种更具吸引力，顺序如下：美国白蛾＞棉铃虫＞甜菜夜蛾 = 草地贪夜蛾。天然宿主的蛹含有1-十二碳烯，而两种天然非宿主不产生这种物质。当基于蛹释放的物种特异性混合物与最佳混合物之间的差异制成的“引诱剂”喷洒在天然非宿主蛹上时，它们显著提高了白蛾周氏啮小蜂对非宿主蛹的吸引力。
这些结果表明，宿主产生的特定挥发性化合物引导白蛾周氏啮小蜂区分天然宿主和非宿主。总体而言，本研究为制定行为改变策略以重新引导白蛾周氏啮小蜂的攻击来控制重要的非宿主害虫奠定了基础。© 2023 化学工业协会。
