Anderson R R, Lee T T, Saewert D C, Sowden K M, Valkirs G E
Clin Chem. 1986 Sep;32(9):1692-5.
We have developed a method for incorporating an internal reference zone in addition to the test zone in a single immunoConcentration device. We have illustrated the method by constructing an internally referenced assay for human choriogonadotropin (HCG) in serum. The internally referenced assay, which can be performed in about 5 min, provides a measured overall precision (CV) of approximately 15% at a concentration, in serum, of approximately 50 int. units/L. The test zone consists of an anti-HCG antibody adsorbed onto latex microspheres and entrapped within a porous support. The reference zone contains antibody against alkaline phosphatase (EC 3.1.3.1), which has also been adsorbed onto latex microspheres and trapped within the support. The use of an internal reference moderates the effects of variations in assay conditions on the interpretation of assay results.
我们开发了一种方法，可在单个免疫浓缩装置中除测试区外并入一个内部参考区。我们通过构建血清中人绒毛膜促性腺激素（HCG）的内部参考测定法来说明该方法。这种内部参考测定法可在约5分钟内完成，在血清中浓度约为50国际单位/升时，测得的总精密度（CV）约为15%。测试区由吸附在乳胶微球上并截留在多孔支持物内的抗HCG抗体组成。参考区含有抗碱性磷酸酶（EC 3.1.3.1）的抗体，该抗体也已吸附在乳胶微球上并截留在支持物内。使用内部参考可减轻测定条件变化对测定结果解释的影响。
