Bock J L, Furgiuele J, Segen J C
Clin Chem. 1985 Mar;31(3):441-4.
We evaluated the Hybritech Tandem-E procedure for quantifying choriogonadotropin (hCG) in human serum. In this "sandwich"-type assay, two monoclonal antibodies directed against different regions of the hCG molecule are used, one coated on a plastic bead, the second conjugated to alkaline phosphatase. The assay can detect as little as 1.0 int. unit of the hormone per liter, shows a linear response up to at least 200 int. units/L, and has good precision. By prolonging the incubations for formation of the sandwich and for substrate hydrolysis, one can achieve higher sensitivity at the expense of a narrower linear range. Correlation with a conventional radioimmunoassay for the beta subunit of hCG was generally excellent, but in one instance the Tandem-E gave an apparently false positive result.
我们评估了Hybritech Tandem-E法在定量检测人血清中绒毛膜促性腺激素（hCG）方面的性能。在这种“夹心”式检测中，使用了两种针对hCG分子不同区域的单克隆抗体，一种包被在塑料珠上，另一种与碱性磷酸酶偶联。该检测方法每升能检测低至1.0国际单位的激素，在至少200国际单位/升的范围内呈线性反应，并且具有良好的精密度。通过延长夹心形成和底物水解的孵育时间，可以以较窄的线性范围为代价实现更高的灵敏度。与传统的hCGβ亚基放射免疫分析的相关性总体良好，但在一个实例中，Tandem-E法给出了明显的假阳性结果。
