Cham B E, Roeser P, Nikles A C, Ridgway K M
Clin Chim Acta. 1986 Jul 15;158(1):71-9. doi: 10.1016/0009-8981(86)90117-8.
Delipidation of liver homogenates, using an organic solvent system which does not denature proteins, increases measurable ferritin by 25-33%, compared to ferritin concentrations by standard heat supernatant assays. When applied to polyacrylamide gradient gels, lipid-associated ferritin does not enter the gel but after delipidation, this ferritin co-migrates with cytosolic ferritin and with purified liver ferritin. The biological significance of the association of ferritin with lipid has yet to be examined.
使用不会使蛋白质变性的有机溶剂系统对肝脏匀浆进行脱脂处理后，与标准热上清液分析法测得的铁蛋白浓度相比，可测量的铁蛋白增加了25% - 33%。当应用于聚丙烯酰胺梯度凝胶时，与脂质相关的铁蛋白不会进入凝胶，但脱脂处理后，这种铁蛋白会与胞质铁蛋白以及纯化的肝脏铁蛋白共同迁移。铁蛋白与脂质结合的生物学意义尚待研究。
